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Titolo:
Effect of temperature on growth, hemagglutination, and protease activity of Porphyromonas gingivalis
Autore:
Percival, RS; Marsh, PD; Devine, DA; Rangarajan, M; Aduse-Opoku, J; Shepherd, P; Curtis, MA;
Indirizzi:
Leeds Dent Inst, Div Oral Biol, Leeds LS2 9LU, W Yorkshire, England Leeds Dent Inst Leeds W Yorkshire England LS2 9LU U, W Yorkshire, England Univ London Queen Mary & Westfield Coll, St Bartholomews & Royal London Sch Univ London Queen Mary & Westfield Coll London England Royal London Sch UnitedEnglandDent Sch Guys & St Thomas Hosp, Dept Immunol, London SE1 9RT,United Med & Dent Sch Guys & St Thomas Hosp London England SE1 9RT 9RT,
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 4, volume: 67, anno: 1999,
pagine: 1917 - 1921
SICI:
0019-9567(199904)67:4<1917:EOTOGH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTEROIDES-GINGIVALIS; CYSTEINE PROTEINASE; PERIODONTITIS; W50; EXPRESSION; DISEASE; PRPRI; PATHOGENESIS; ANTIBODIES; PRECURSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Marsh, PD LeedsEnglandnst, Div Oral Biol, Clarendon Way, Leeds LS2 9LU, W Yorkshire, Leeds Dent Inst Clarendon Way Leeds W Yorkshire England LS2 9LU
Citazione:
R.S. Percival et al., "Effect of temperature on growth, hemagglutination, and protease activity of Porphyromonas gingivalis", INFEC IMMUN, 67(4), 1999, pp. 1917-1921

Abstract

Bacteria persisting in periodontal pockets are exposed to elevated temperatures during periods of inflammation, Temperature is an environmental factor that can modulate gene expression. Consequently, in the present study we examined the effect of temperature on the expression of virulence determinants by the periodontopathogen, Porphyromonas gingivalis, P. gingivalis W50 was grown in a complex medium under hemin excess at pH 7.0 and at a constant temperature of either 37, 39, or 41 degrees C; cultures were monitored for protease and hemagglutinin activity. P. gingivalis grew well at all threetemperatures. An increase in growth temperature from 37 to 39 degrees C resulted in a 65% reduction in both total arginine- and lysine-specific activities (P < 0.01). A further rise in growth temperature to 41 degrees C led to even greater reductions in arginine-specific (82%; P < 0.001) and lysine-specific (73%; P < 0.01) activities. These reductions were also associated,vith an altered distribution of individual arginine-specific enzyme isoforms, At 41 degrees C, there was a disproportionate reduction in the level ofthe heterodimeric RI protease, which also contains adhesin domains. The reduction also correlated with a markedly diminished hemagglutination activity of cells, especially in those grown at 41 degrees C, and a reduced immunoreactivity,vith a monoclonal antibody which recognizes gene products involved in hemagglutination, Thus, as the environmental temperature increased, P. gingivalis adopted a less aggressive phenotype, while retaining cell population levels, The coordinate down-regulation of virulence gene expression in response to an environmental cue linked to the intensity of the host inflammatory response is consistent with the clinically observed cyclical nature of disease progression in periodontal diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:45:07