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Titolo:
Bacillus subtilis alpha-amylase: the rate limiting step of secretion is growth phase-independent
Autore:
Haddaoui, E; Chambert, R; Petit-Glatron, MF; Lindy, O; Sarvas, M;
Indirizzi:
Univisaris 06, CNRS, Inst Jacques Monod, Lab Genet & Membranes, F-75251 Par Univ Paris 06 Paris France 05 Monod, Lab Genet & Membranes, F-75251 Par Univis,ris 07, CNRS, Inst Jacques Monod, Lab Genet & Membranes, F-75251 Par Univ Paris 07 Paris France F-75251 d, Lab Genet & Membranes, F-75251 Par Natl Publ Hlth Inst, Lab Vaccine Dev, FIN-00300 Helsinki, Finland Natl Publ Hlth Inst Helsinki Finland FIN-00300 N-00300 Helsinki, Finland
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 1, volume: 173, anno: 1999,
pagine: 127 - 131
SICI:
0378-1097(19990401)173:1<127:BSATRL>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN SECRETION; LEVANSUCRASE; MECHANISM; EXPORT; STAGE; PRSA;
Keywords:
Bacillus subtilis; protein secretion; alpha-amylase; stationary phase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Chambert, R UnivlParis 06, CNRS, Inst Jacques Monod, Lab Genet & Membranes, Tour 43,2 P Univ Paris 06 Tour 43,2 Pl Jussieu Paris France 05 our 43,2 P
Citazione:
E. Haddaoui et al., "Bacillus subtilis alpha-amylase: the rate limiting step of secretion is growth phase-independent", FEMS MICROB, 173(1), 1999, pp. 127-131

Abstract

When Bacillus subtilis alpha-amylase was expressed under the control of sacR in a degU32(Hy) strain, the production of exoenzyme occurred during boththe exponential and stationary phases of growth. In each phase, pulse-chase experiments showed that the rate-limiting step of the secretion process was the release of the processed form of the protein in each physiological context. The rate of this event was slightly slower (t(1/2)=3.2 min) during the stationary phase than during the exponential phase (t(1/2)=2 min). The effecters which possibly control the efficiency of the release stage, the level of PrsA or the calcium binding properties of the cell wall, remained unchanged throughout growth phases. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 13:57:41