Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
High bilirubin levels interfere with serum tartrate-resistant acid phosphatase determination: Relevance as a marker of bone resorption in jaundiced patients
Autore:
Alvarez, L; Peris, P; Bedini, JL; Pares, A; Monegal, A; Guanabens, N; Mas, E; Aibar, C; Ballesta, AM;
Indirizzi:
Univ Barcelona, Hosp Clin, Serv Biochem, Barcelona 08036, Spain Univ Barcelona Barcelona Spain 08036 erv Biochem, Barcelona 08036, Spain Univ Barcelona, Hosp Clin, Serv Rheumatol, Barcelona 08036, Spain Univ Barcelona Barcelona Spain 08036 v Rheumatol, Barcelona 08036, Spain Univ Barcelona, Hosp Clin, Metab Bone Dis Unit, Barcelona 08036, Spain Univ Barcelona Barcelona Spain 08036 ne Dis Unit, Barcelona 08036, Spain Univ Barcelona, Hosp Clin, Liver Unit, Barcelona 08036, Spain Univ Barcelona Barcelona Spain 08036 Liver Unit, Barcelona 08036, Spain
Titolo Testata:
CALCIFIED TISSUE INTERNATIONAL
fascicolo: 4, volume: 64, anno: 1999,
pagine: 301 - 303
SICI:
0171-967X(199904)64:4<301:HBLIWS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALKALINE-PHOSPHATASE; IMMUNOASSAY; DISEASE; ISOENZYME;
Keywords:
hepatic diseases; biochemical markers; bone turnover; acid phosphatase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Alvarez, L Univ Barcelona, Hosp Clin, Serv Biochem, C Villarroel 170, Barcelona 08036, Univ Barcelona C Villarroel 170 Barcelona Spain 08036 a 08036,
Citazione:
L. Alvarez et al., "High bilirubin levels interfere with serum tartrate-resistant acid phosphatase determination: Relevance as a marker of bone resorption in jaundiced patients", CALCIF TIS, 64(4), 1999, pp. 301-303

Abstract

Serum tartrate-resistant acid phosphatase (TRAcP) activity is considered to be a biochemical marker of bone resorption. Recently, a lack of specificity of collagen-related markers for assessing bone turnover has been observed in patients with chronic liver disease. Thus, it could be of great interest to determine serum TRAcP activity in such patients. However, nonspecificity of the analytical reaction could occur when hemolyzed, lipemic, or icteric specimens are analyzed. Therefore, we have studied the interference caused by bilirubin in the measurement of serum TRAcP activity using the Hillmann method. The interference was assessed in two pools of serum containing different bilirubin concentrations but with similar total AcP levels. Mixing proportional parts of the two pools, 10 samples were also obtained. Serumactivities of total AcP and TRAcP, and the concentration of bilirubin weremeasured in the 10 samples. Both the actual and the expected values obtained by theoretical calculations were compared. Serum bilirubin values of 2.4mg/dl showed a negative interference of 15% in the determination of serum TRAcP activity, whereas values of bilirubin higher than 10 mg/dl interferedtotally with the measurement of serum TRAcP. Bilirubin did not interfere with the total AcP determination. This study clearly shows the interference of bilirubin in the determination of serum TRAcP. This finding should be considered when bone metabolism disorders are evaluated in jaundiced patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:38:35