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Titolo:
Predictive control of hollow-fiber bioreactors for the production of monoclonal antibodies
Autore:
Dowd, JE; Weber, I; Rodriguez, B; Piret, JM; Kwok, KE;
Indirizzi:
Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1Z3, Canada Univ British Columbia Vancouver BC Canada V6T 1Z3 ver, BC V6T 1Z3, Canada Univ British Columbia, Dept Chem & Bioresource Engn, Vancouver, BC V5Z 1M9, Univ British Columbia Vancouver BC Canada V5Z 1M9 Vancouver, BC V5Z 1M9, INSA Toulouse, Dept Genie Biochim & Alimentaire, Toulouse, France INSA Toulouse Toulouse France e Biochim & Alimentaire, Toulouse, France StemCell Technol Inc, Vancouver, BC, Canada StemCell Technol Inc Vancouver BC Canada hnol Inc, Vancouver, BC, Canada
Titolo Testata:
BIOTECHNOLOGY AND BIOENGINEERING
fascicolo: 4, volume: 63, anno: 1999,
pagine: 484 - 492
SICI:
0006-3592(19990520)63:4<484:PCOHBF>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MODEL ADAPTIVE-CONTROL; FED-BATCH FERMENTATION; CELL-CULTURE; PRESSURE; GLUCOSE; CULTIVATION; DENSITY; SYSTEMS; GROWTH;
Keywords:
predictive control; hollow-fiber bioreactors; monoclonal antibodies; perfusion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Piret, JM Univd,ritish Columbia, Biotechnol Lab, Room 237,Wesbrook Bldg,6174 Univ Blv Univ British Columbia Room 237,Wesbrook Bldg,6174 Univ Blvd Vancouver BC Canada V6T 1Z3
Citazione:
J.E. Dowd et al., "Predictive control of hollow-fiber bioreactors for the production of monoclonal antibodies", BIOTECH BIO, 63(4), 1999, pp. 484-492

Abstract

The selection of medium feed rates for perfusion bioreactors represents a challenge for process optimization, particularly in bioreactors that are sampled infrequently. When the present and immediate future of a bioprocess can be adequately described, predictive control can minimize deviations fromset points in a manner that can maximize process consistency. Predictive control of perfusion hollow-fiber bioreactors was investigated in a series of hybridoma cell cultures that compared operator control to computer estimation of feed rates. Adaptive software routines were developed to estimate the current and predict the future glucose uptake and lactate production of the bioprocess at each sampling interval. The current and future glucose uptake rates were used to select the perfusion feed rate in a designed response to deviations from the set point values. The routines presented a graphical user interface through which the operator was able to view the up-to-date culture performance and assess the model description of the immediate future culture performance. In addition, fewer samples were taken in the computer-estimated cultures, reducing labor and analytical expense. The use of these predictive controller routines and the graphical user interface decreased the glucose and lactate concentration variances up to sevenfold, and antibody yields increased by 10% to 43%. (C) 1999 John Wiley & Sons, Inc.

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Documento generato il 02/07/20 alle ore 22:25:28