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Titolo:
Monocyte chemotactic protein-1 gene and protein expression in atherogenesis of hypercholesterolemic rabbits
Autore:
Chen, YL; Chang, YJ; Jiang, MJ;
Indirizzi:
Natl Cheng Kung Univ, Coll Med, Dept Anat, Tainan 70101, Taiwan Natl ChengKung Univ Tainan Taiwan 70101 Dept Anat, Tainan 70101, Taiwan Natl Yang Ming Med Univ, Inst Anat, Taipei 11221, Taiwan Natl Yang Ming Med Univ Taipei Taiwan 11221 t Anat, Taipei 11221, Taiwan
Titolo Testata:
ATHEROSCLEROSIS
fascicolo: 1, volume: 143, anno: 1999,
pagine: 115 - 123
SICI:
0021-9150(199903)143:1<115:MCPGAP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; LOW-DENSITY-LIPOPROTEIN; BLOOD MONONUCLEAR LEUKOCYTES; HUMAN ENDOTHELIAL-CELLS; AMINO-ACID ANALYSIS; CHEMOATTRACTANT PROTEIN-1; ATHEROSCLEROTIC LESIONS; NONHUMAN PRIMATE; FATTY STREAK; PURIFICATION;
Keywords:
atherogenesis; monocyte chemotactic protein-1; in situ hybridization; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Jiang, MJ Natl Cheng Kung Univ, Coll Med, Dept Anat, Tainan 70101, Taiwan Natl Cheng Kung Univ Tainan Taiwan 70101 Tainan 70101, Taiwan
Citazione:
Y.L. Chen et al., "Monocyte chemotactic protein-1 gene and protein expression in atherogenesis of hypercholesterolemic rabbits", ATHEROSCLER, 143(1), 1999, pp. 115-123

Abstract

Monocyte adherence to the endothelium and subsequent migration into the subendothelial space are important early events in atherogenesis. Monocyte chemotactic protein-1 (MCP-1) has been shown to be highly expressed in both human atheroma and advanced atherosclerotic lesions of experimental animals. To establish the temporal correlation between MCP-1 expression and plaque development, we examined the expression of MCP-1 during atherogenesis of hypercholesterolemic rabbits using Northern blot analysis, in situ hybridization, and immunohistochemistry. New Zealand White rabbits were fed with 2% cholesterol-containing diet for 1 day, 3 days, 1 week, 3 weeks or 6 weeks. The plasma levels of total cholesterol were significantly increased 3 days after cholesterol feeding and continued to increase during the entire cholesterol-feeding period. Northern blot analysis showed that MCP-1 mRNA levels remained unchanged following cholesterol feeding for up to 1 week, were higher than control levels at 3-week and increased even higher at 6-week. In situ hybridization showed that after 3 weeks of cholesterol feeding, MCP-1 mRNA expression was up-regulated in newly-formed fatty streaks and parts of tunica media in the presence or absence of fatty streaks. At 6-week, pronounced MCP-1 mRNA expression was detected with similar distribution. In contrast, MCP-1 mRNA was detected only in a few endothelial cells and adventitiain control and experimental groups feeding cholesterol up to 1-week. Immunostaining of serial sections indicated that MCP-1 was expressed by macrophages and smooth muscle cells in rabbits fed with cholesterol for 3 or 6 weeks. No MCP-1 was detected in intima or media in all other groups. These results show that a lag period exists between serum cholesterol increase and upregulation of MCP-I expression, suggesting that cholesterol modifications (e.g. oxidation) are required to stimulate MCP-1 expression. In addition, MCP-1 expressed by both macrophages and smooth muscle cells during the initial stages of atherosclerosis is likely to contribute to the development of fatty streaks in hypercholesterolemic rabbits. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:11:36