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Titolo:
MG2-DEPENDENT ENDONUCLEASE ACTIVITIES OF HUMAN MYELOID-LEUKEMIA CELLSCAPABLE OF PRODUCING NUCLEOSOMAL-SIZE DNA FRAGMENTATION( OR MN2+)
Autore:
KAWABATA H; ANZAI N; MASUTANI H; HIRAMA T; HISHITA T; DODO M; MASUDA T; YOSHIDA Y; OKUMA M;
Indirizzi:
KYOTO UNIV,CTR S E ASIAN STUDIES SAKYO KYOTO 606 JAPAN KYOTO UNIV,CTR S E ASIAN STUDIES SAKYO KYOTO 606 JAPAN KYOTO UNIV,INST IMMUNOL SAKYO KYOTO 606 JAPAN KYOTO UNIV,INST VIRUS RES SAKYO KYOTO 606 JAPAN KYOTO UNIV,GRAD SCH MED,DEPT HEMATOL & ONCOL SAKYO KYOTO 606 JAPAN
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 1, volume: 233, anno: 1997,
pagine: 133 - 138
SICI:
0006-291X(1997)233:1<133:MEAOHM>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
APOPTOTIC RAT THYMOCYTES; IN-VITRO; IDENTIFICATION; DEOXYRIBONUCLEASE; ACTIVATION; ELEVATIONS; CLEAVAGE; SUICIDE; DEATH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
H. Kawabata et al., "MG2-DEPENDENT ENDONUCLEASE ACTIVITIES OF HUMAN MYELOID-LEUKEMIA CELLSCAPABLE OF PRODUCING NUCLEOSOMAL-SIZE DNA FRAGMENTATION( OR MN2+)", Biochemical and biophysical research communications, 233(1), 1997, pp. 133-138

Abstract

The presence of at least two distinct Mg2+- or Mn2+- dependent, Ca2+-independent endonuclease activities was shown in the myeloid leukemia cell line P39. One of them was recovered from nuclear extracts and theother from a cytoplasmic fraction. The molecular size of the former was 30 kDa in both gel filtration and activity gel and that of the latter approximately 130-140 kDa in gel filtration and 65-70 kDa in activity gel. These two activities were almost completely inhibited by 0.1 mM ZnCl2 or 0.1 mM aurintricarboxylic acid, common inhibitors of apoptosis. Both could produce nucleosomal-size DNA fragmentation when incubated with diethyl-pyrocarbonate-treated nuclei as substrates, and the pattern of cleavage was 3'-OH and 5'-P. Taken together, either or both of these activities may be associated with apoptosis of myeloid leukemia cells. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 12:54:49