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Titolo:
Detection of Leishmania braziliensis in naturally infected individual sandflies by the polymerase chain reaction
Autore:
Rodriguez, N; Aguilar, CM; Barrios, MA; Barker, DC;
Indirizzi:
Cent Univ Venezuela, Inst Biomed, Caracas 1010A, Venezuela Cent Univ Venezuela Caracas Venezuela 1010A ed, Caracas 1010A, Venezuela Univ Cambridge, Cambridge, England Univ Cambridge Cambridge EnglandUniv Cambridge, Cambridge, England
Titolo Testata:
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
fascicolo: 1, volume: 93, anno: 1999,
pagine: 47 - 49
SICI:
0035-9203(199901/02)93:1<47:DOLBIN>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINETOPLAST DNA; DIAGNOSIS; VENEZUELA; COMPLEX;
Keywords:
sandflies; Leishmania braziliensis; polymerase chain reaction; DNA hybridization; vectors; Lutzomyia gomezi; Lutzomyia panamensis; Venezuela;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Rodriguez, N Cent Univ Venezuela, Inst Biomed, Apdo 4043, Caracas 1010A, Venezuela Cent Univ Venezuela Apdo 4043 Caracas Venezuela 1010A ezuela
Citazione:
N. Rodriguez et al., "Detection of Leishmania braziliensis in naturally infected individual sandflies by the polymerase chain reaction", T RS TROP M, 93(1), 1999, pp. 47-49

Abstract

The natural infection of sandflies by Leishmania in wild-caught specimens was studied, using the polymerase chain reaction (PCR)-hybridization technique. The PCR was carried out using 2 oligonucleotides (primers 3J1 and 3J2)derived from a repetitive nuclear DNA sequence. The primers support the enzymatic amplification of a fragment of approximately 500 bp, present in thenuclear DNA of Leishmania braziliensis. The expected band was observed in 5 of 65 sandflies containing flagellates. After hybridization with a species-specific probe, we confirmed natural infection by L. braziliensis. The technique allowed the identification of Lutzomyia gomezi and Lu. panamensis as vectors of L. braziliensis in an endemic area of cutaneous leishmaniasis in Urama, Puerto Cabello district in Venezuela. As far as we are aware, this work constitutes the first report of natural infection of Lu. panamensis with L. braziliensis in the study area. We also demonstrate that PCR-hybridization is a suitable approach to establish the Leishmania-sandfly relationship and will be useful in epidemiological studies of leishmaniasis in endemic areas.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 19:25:58