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Titolo:
Ectopic expression of constitutively activated Ral GTPase inhibits cell shape changes during Drosophila eye development
Autore:
Sawamoto, K; Yamada, C; Kishida, S; Hirota, Y; Taguchi, A; Kikuchi, A; Okano, H;
Indirizzi:
OsakaJapan, Sch Med, Biomed Res Ctr, Dept Neuroanat, Suita, Osaka 5650871,Osaka Univ Suita Osaka Japan 5650871 ept Neuroanat, Suita, Osaka 5650871, Hiroshima Univ, Sch Med, Dept Biochem, Minami Ku, Hiroshima 7340037, JapanHiroshima Univ Hiroshima Japan 7340037 nami Ku, Hiroshima 7340037, Japan JST, CREST, Minato Ku, Tokyo 1050011, Japan JST Tokyo Japan 1050011JST, CREST, Minato Ku, Tokyo 1050011, Japan
Titolo Testata:
ONCOGENE
fascicolo: 11, volume: 18, anno: 1999,
pagine: 1967 - 1974
SICI:
0950-9232(19990318)18:11<1967:EEOCAR>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE DISSOCIATION STIMULATOR; PROTEIN TYROSINE KINASE; C-FOS PROMOTER; PUTATIVE EFFECTOR; FATE; MEMBRANE; GROWTH; IDENTIFICATION; TRANSFORMATION; MELANOGASTER;
Keywords:
actin; cell shape; Drosophila; Ra1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Okano, H OsakaOsaka, Sch Med, Biomed Res Ctr, Dept Neuroanat, 2-2 Yamadaoka, Suita, Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 adaoka, Suita,
Citazione:
K. Sawamoto et al., "Ectopic expression of constitutively activated Ral GTPase inhibits cell shape changes during Drosophila eye development", ONCOGENE, 18(11), 1999, pp. 1967-1974

Abstract

The small GTP-binding protein Ral is activated by RalGDS, one of the effector molecules for Ras. Active Ral binds to a GTPase activating protein for CDC42 and Rac. Although previous studies suggest a role for Ral in the regulation of CDC42 and Rac, which are involved in arranging the cytoskeleton, its in vivo function is largely unknown. To examine the effect of overexpressing Ral on development, transgenic Drosophila were generated that overexpress wild-type or mutated Ral during eye development. While wild-type Ral caused no developmental defects, expression of a constitutively activated protein resulted in a rough eye phenotype, Activated Ral did not affect cell fate determination in the larval eye discs but caused severe disruption of the ommatidial organization later in pupal development. Phalloidin stainingshowed that activated Ral perturbed the cytoskeletal structure and cell shape changes during pupal development. This phenotype is similar to that caused by RhoA overexpression. In addition, the phenotype was synergistically enhanced by the coexpression of RhoA, These results suggest that Ral functions to control the cytoskeletal structure required for cell shape changes during Drosophila development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:56:50