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Titolo:
Retinoic acids up-regulate steroidogenic acute regulatory protein gene
Autore:
Lee, HK; Yoo, MS; Choi, HS; Kwon, HB; Soh, J;
Indirizzi:
Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonnam Natl Univ, Coll Nat Sci, Dept Biol, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 148, anno: 1999,
pagine: 1 - 10
SICI:
0303-7207(19990225)148:1-2<1:RAUSAR>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHAIN CLEAVAGE CYTOCHROME-P-450; LEYDIG TUMOR-CELLS; RECEPTOR-ALPHA; VITAMIN-A; TESTOSTERONE PRODUCTION; RAT TESTIS; EXPRESSION; BINDING; DNA; STAR;
Keywords:
steroidogenic acute regulatory protein; StAR; gene expression; retinoic acids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Soh, J Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 500757, South Korea
Citazione:
H.K. Lee et al., "Retinoic acids up-regulate steroidogenic acute regulatory protein gene", MOL C ENDOC, 148(1-2), 1999, pp. 1-10

Abstract

The steroidogenic acute regulatory (StAR) protein plays essential roles inthe delivery of cytosolic cholesterol into the mitochondrial inner membrane, which is an acute regulated and rate-limiting step for the steroid hormone synthesis. Since retinoic acids (RAs) are known to induce the synthesis of steroid hormones in mouse Leydig cells in vitro, mouse Leydig tumour cells, K28, were used to determine the effect of RAs on the level of StAR mRNAby Northern blot analysis. The level of StAR mRNA reached the maximum in a4-8 h treatment with all-trans-RA (atRA) or 9-cis-RA (9cRA), and the effects were dose-dependent. The effect of 9cRA on the levels of StAR mRNA was blocked by actinomycin D, which indicates that 9cRA might exert a direct effect on the transcription of the gene. Promoter/reporter constructs containing a 5'-flanking region of the mouse or rat StAR gene were prepared, and luciferase activity was assayed following transient transfection into K28 or adrenal tumour cells, Y1. The result revealed that the luciferase activity was increased by 4-5-fold in response to the treatment of 9cRA, which indicated that 9cRA participates transcriptional activation of the StAR protein gene. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

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Documento generato il 03/12/20 alle ore 21:51:38