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Titolo:
Changes in the noninvasive, in vivo electrical impedance of three xenografts during the necrotic cell-response sequence
Autore:
McRae, DA; Esrick, MA; Mueller, SC;
Indirizzi:
Georgetown Univ, Dept Radiat Med, Rockville, MD 20850 USA Georgetown UnivRockville MD USA 20850 adiat Med, Rockville, MD 20850 USA Georgetown Univ, Dept Phys, Washington, DC 20057 USA Georgetown Univ Washington DC USA 20057 pt Phys, Washington, DC 20057 USA Georgetown Univ, Dept Cell Biol, Washington, DC 20057 USA Georgetown UnivWashington DC USA 20057 ll Biol, Washington, DC 20057 USA Georgetown0007v, Med Ctr, Vincent T Lombardi Canc Res Ctr, Washington, DC 2 Georgetown Univ Washington DC USA 20007 di Canc Res Ctr, Washington, DC 2
Titolo Testata:
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
fascicolo: 4, volume: 43, anno: 1999,
pagine: 849 - 857
SICI:
0360-3016(19990301)43:4<849:CITNIV>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCISED EMT-6 TUMORS; DIELECTRIC-PROPERTIES; MILD HYPERTHERMIA; APOPTOSIS; MORPHOLOGY; TISSUE; INJURY; DEATH; SKIN;
Keywords:
electrical impedance spectroscopy; resistivity; tumor; necrosis; in vivo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: McRae, DA GeorgetownUSAiv, Dept Radiat Med, 9711 Med Ctr Dr 111, Rockville, MD 20850 Georgetown Univ 9711 Med Ctr Dr 111 Rockville MD USA 20850 20850
Citazione:
D.A. McRae et al., "Changes in the noninvasive, in vivo electrical impedance of three xenografts during the necrotic cell-response sequence", INT J RAD O, 43(4), 1999, pp. 849-857

Abstract

Purpose: To investigate the noninvasive, in vivo use of electrical impedance spectroscopy (EIS) as a method for observing the real-time, cellular-level responses of a volume of tissue to therapies. Here, we studied the EIS response during the development and progression of hyperthermia-induced coagulative necrosis in three diverse human xenografts,Methods and Materials: A necrotic cell response sequence was selectively induced in three types of subcutaneously-grown human tumor xenografts by applying hyperthermia at 44.5 degrees C. The electrical impedance of the tumors was measured from 100 Hz to 10 MHZ, noninvasively, in vivo during the treatments. From the full spectrum EIS, ratios between resistivities at selected frequencies (rho-ratios) were used as indicators of the changes in the electrical impedance spectra of each tumor's cell population. Results: The rho-ratios consistently demonstrated characteristic, early, rapid increases which coincided with cell and organelle swelling typical of early necrosis, These increases subsequently slowed, but no decrease began before the end of treatment, unlike previous, similarly treated, thermo-sensitive EMT6 mouse tumors. This was consistent with the xenograft histology,which revealed ubiquitous, early-stage coagulative necrosis, with no grossplasma membrane damage at the end of treatment. The extent of both the necrosis and rho-ratio changes were similar to those seen early in the EMT6 tumor treatment. Within several days after treatment, the xenograft volumes regressed nearly completely, suggesting completion of the cell populations' necrotic response (lysing) during this period. Consistent with this, extended EIS measurements over a 24-h posttreatment period allowed tracking of the necrotic response sequence through this lysing phase for one type of xenograft,Conclusion: The change in the electrical impedance of a volume of tumor tissue which occurs during and/or after a hyperthermia treatment can be correlated with the extent of necrosis observed histologically in the cell population. (C) 1999 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:50:44