Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Reconstitution of an electrogenic auxin transport activity mediated by Arabidopsis thaliana plasma membrane proteins
Autore:
Szponarski, W; Guibal, O; Espuna, M; Doumas, P; Rossignol, M; Gibrat, R;
Indirizzi:
INRA ENSA M CNRS, URA 2133, F-34060 Montpellier, France INRA ENSA M CNRS Montpellier France F-34060 F-34060 Montpellier, France
Titolo Testata:
FEBS LETTERS
fascicolo: 1, volume: 446, anno: 1999,
pagine: 153 - 156
SICI:
0014-5793(19990305)446:1<153:ROAEAT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CUCURBITA-PEPO L; N-1-NAPHTHYLPHTHALAMIC ACID; HYPOCOTYL SEGMENTS; CORN COLEOPTILES; IN-VITRO; VESICLES; BINDING; INHIBITORS; CARRIERS; PROBES;
Keywords:
auxin transport; efflux carrier; plasma membrane; protein reconstitution; diffusion potential; fluorescent probe; Arabidopsis thaliana;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Szponarski, W INRA ENSA M CNRS, URA 2133, Pl Viala, F-34060 Montpellier, France INRA ENSA M CNRS Pl Viala Montpellier France F-34060 France
Citazione:
W. Szponarski et al., "Reconstitution of an electrogenic auxin transport activity mediated by Arabidopsis thaliana plasma membrane proteins", FEBS LETTER, 446(1), 1999, pp. 153-156

Abstract

Plasma membrane proteins from Arabidopsis thaliana leaves were reconstituted into proteoliposomes and a K+ diffusion potential was generated. The resulting ionic fluxes, determined in the presence of the plant hormone auxin (indole-3 acetic acid), showed an additional electrogenic and saturable component, with a K-M of 6 mu M. This flux was neither detected in liposomes in the presence of indole-3 acetic acid, nor in proteoliposomes in the presence of an inactive auxin analog and was completely inhibited by 3 mu M naphtylphthalamic acid, a specific inhibitor of the auxin efflux carrier. The efficiency of the reconstituted carrier and the mechanism of its regulation by naphtylphthalamic acid are discussed. (C) 1999 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 10:33:28