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Titolo:
CT-guided intratumoral gene therapy in non-small-cell lung cancer
Autore:
Kauczor, HU; Schuler, M; Heussel, CP; von Weymarn, A; Bongartz, G; Rochlitz, C; Huber, C; Thelen, M;
Indirizzi:
Univ Mainz, Dept Radiol, D-55131 Mainz, Germany Univ Mainz Mainz GermanyD-55131 nz, Dept Radiol, D-55131 Mainz, Germany Univ Mainz, Dept Med 3, D-55131 Mainz, Germany Univ Mainz Mainz Germany D-55131 inz, Dept Med 3, D-55131 Mainz, Germany Univ Basel, Kantonsspital, Inst Diagnost Radiol, CH-4031 Basel, Switzerland Univ Basel Basel Switzerland CH-4031 Radiol, CH-4031 Basel, Switzerland Univ Basel, Kantonsspital, Div Oncol, Dept Med, CH-4031 Basel, SwitzerlandUniv Basel Basel Switzerland CH-4031 ept Med, CH-4031 Basel, Switzerland
Titolo Testata:
EUROPEAN RADIOLOGY
fascicolo: 2, volume: 9, anno: 1999,
pagine: 292 - 296
SICI:
0938-7994(1999)9:2<292:CIGTIN>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
P53; INJECTION; MELANOMA; ANTIGEN;
Keywords:
computed tomography; therapeutic radiology; gene therapy, p53;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Kauczor, HU Univ Mainz, Dept Radiol, Langenbeckstr 1, D-55131 Mainz, Germany Univ Mainz Langenbeckstr 1 Mainz Germany D-55131 inz, Germany
Citazione:
H.U. Kauczor et al., "CT-guided intratumoral gene therapy in non-small-cell lung cancer", EUR RADIOL, 9(2), 1999, pp. 292-296

Abstract

The objective of this study was to prove the principle of CT-guided gene therapy by intratumoral injection of a tumor suppressor gene as an alternative treatment approach of incurable non-small-cell lung cancer. In a prospective clinical phase I trial six patients with non-small-cell lung cancer and a mutation of the tumor suppressor gene p53 were treated by CT-guided intratumoral gene therapy. Ten milliliters of a vector solution (replication-defective adenovirus with complete wild-type p53 cDNA) were injected under CT guidance. In four cases the vector solution was completely applied to thetumor center, whereas in two cases 2 ml aliquots were injected into different tumor areas. For the procedure the scan room had been approved as a biosafety cabinet. Gene transfer was assessed by reverse transcription and polymerase chain reaction in biopsy specimens obtained under CT guidance 24-48h after therapy. Potential therapeutic efficacy was evaluated on day 28 after treatment using spiral CT. The CT-guided gene therapy! was easily performed in all six patients without intervention-related complications. Besides flu-like symptoms, no significant adverse effects of gene therapy were noted. Three of the four patients with central injection exhibited gene transfer in the posttreatment biopsy. Gene transfer could not be proven in the two patients with multiple 2 ml injections. After 28 days, four of the six patients showed stable disease at the treated tumor site, whereas other tumor manifestations progressed. Computed tomography-guided injections are an adequate and easy-to-perform procedure for intratumoral gene therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 18:43:08