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Titolo:
Translocation of ornithine decarboxylase to the surface membrane during cell activation and transformation
Autore:
Heiskala, M; Zhang, J; Hayashi, S; Holtta, E; Andersson, LC;
Indirizzi:
Haartman Inst, Dept Pathol, FIN-00014 Helsinki, Finland Haartman Inst Helsinki Finland FIN-00014 ol, FIN-00014 Helsinki, Finland Univ Helsinki Hosp, FIN-00014 Helsinki, Finland Univ Helsinki Hosp Helsinki Finland FIN-00014 IN-00014 Helsinki, Finland RW Johnson Pharmaceut Res Inst, San Diego, CA 92121 USA RW Johnson Pharmaceut Res Inst San Diego CA USA 92121 Diego, CA 92121 USA Jikei Univ, Sch Med, Dept Biochem 2, Minato Ku, Tokyo 105, Japan Jikei Univ Tokyo Japan 105 , Dept Biochem 2, Minato Ku, Tokyo 105, Japan Karolinska Inst, Inst Oncol & Pathol, S-17176 Stockholm, Sweden KarolinskaInst Stockholm Sweden S-17176 thol, S-17176 Stockholm, Sweden
Titolo Testata:
EMBO JOURNAL
fascicolo: 5, volume: 18, anno: 1999,
pagine: 1214 - 1222
SICI:
0261-4189(19990301)18:5<1214:TOODTT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY BURST OXIDASE; PROTEIN-KINASE-C; NEUTROPHIL NADPH OXIDASE; GTP-BINDING PROTEINS; FUNCTIONAL DOMAIN; PLASMA-MEMBRANE; EXPRESSION; POLYAMINES; P47(PHOX); PHOSPHORYLATION;
Keywords:
cell activation; membrane targeting; ODC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Heiskala, M Haartman Inst, Dept Pathol, Haartmaninkatu 3, FIN-00014 Helsinki, Finland Haartman Inst Haartmaninkatu 3 Helsinki Finland FIN-00014 and
Citazione:
M. Heiskala et al., "Translocation of ornithine decarboxylase to the surface membrane during cell activation and transformation", EMBO J, 18(5), 1999, pp. 1214-1222

Abstract

Ornithine decarboxylase (ODC) is highly up-regulated in proliferating and transforming cells. Here we show that upon induction, an initial cytosolic increase of ODC is followed by translocation of a fraction of the enzyme tothe surface membrane. ODC membrane translocation is mediated by a p47(phox) membrane-targeting motif-related sequence, as indicated by reduced ODC activity in the membrane fraction of cells treated with a competing, ODC-derived (amino acids 165-172) peptide, RLSVKFGA, which is homologous to the p47(phox) membrane-targeting sequence. p47(phox) membrane translocation is known to be dependent on the phosphorylation of the targeting motif, Analogously, overexpressed ODC,S167A, a mutant ODC lacking the putative phosphorylation site Ser67, is unable to move to the surface membrane. Cells blocked with the RLSVKFGA peptide showed defective transformation, indicating that the motif-mediated translocation of ODC is prerequisite to its biological function. Constitutive targeting of ODC to the membrane using a plasmid encoding the chimeric protein, wild-type ODC with C-terminal linkage to the farnesylation motif of K-ras, caused impaired cytokinesis with an accumulation of polykaryotic cells, Impaired cytokinesis confirms that ODC is involved inmitotic cytoskeletal rearrangement events and pinpoints the importance of relevant membrane targeting to its physiological function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 08:19:00