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Titolo:
Column wall modifications using polyethylene oxide for capillary electrophoresis of ribonucleotides
Autore:
Shao, X; Shen, Y; ONeill, K; Lee, ML;
Indirizzi:
Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA Brigham YoungUniv Provo UT USA 84602 Chem & Biochem, Provo, UT 84602 USA Brigham Young Univ, Dept Microbiol, Provo, UT 84602 USA Brigham Young Univ Provo UT USA 84602 Dept Microbiol, Provo, UT 84602 USA
Titolo Testata:
CHROMATOGRAPHIA
fascicolo: 5-6, volume: 49, anno: 1999,
pagine: 299 - 305
SICI:
0009-5893(199903)49:5-6<299:CWMUPO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUSED-SILICA COLUMNS; ZONE ELECTROPHORESIS; NUCLEOTIDE POOLS; PROTEINS; CHROMATOGRAPHY; ADSORPTION; SEPARATION; BUFFERS; CELLS;
Keywords:
capillary electrophoresis; column technology; deactivation; polyethyleneoxides; ribonucleotides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, ML Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA Brigham Young Univ Provo UT USA 84602 iochem, Provo, UT 84602 USA
Citazione:
X. Shao et al., "Column wall modifications using polyethylene oxide for capillary electrophoresis of ribonucleotides", CHROMATOGR, 49(5-6), 1999, pp. 299-305

Abstract

In this study, a variety of fused silica capillaries with different combinations and sequences of treatments with HMDS and polyethylene oxide were prepared in order to develop an optimized column modification method for analysis of ribonucleotides. The 12 most common ribonucleotides (UTP, CTP, ATP,GTP, UDP, CDP, ADP, GDP, UMP, CMP, AMP, and GMP) in human cells were used as test solutes. Column performance measurements, including electroosmotic flow (EOF), solute migration speed and retention, column efficiency, peak shape, and resolution were investigated. By analyzing solute migration speedand retention of various hydrophilic/hydrophobic solutes, the column wall effects (EOF and adsorption) can be distinguished. This analysis method cangive guidance in optimizing polymer coating properties (hydrophilicity/hydrophobicity) for CE columns. By studying the performance of these columns after various surface treatments, we were able to improve the separation of ribonucleotides from real samples to within 16 minutes with high efficiencyand stability (over 300 analyses) using columns first deactivated with hexamethyldisilazane, and then coated with polyethylene oxide.

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Documento generato il 28/09/20 alle ore 12:10:27