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Titolo:
Probing ribosome structure by europium-induced RNA cleavage
Autore:
Dorner, S; Barta, A;
Indirizzi:
Univ Vienna, Vienna Bioctr, Inst Biochem, A-1030 Vienna, Austria Univ Vienna Vienna Austria A-1030 , Inst Biochem, A-1030 Vienna, Austria
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 2, volume: 380, anno: 1999,
pagine: 243 - 251
SICI:
1431-6730(199902)380:2<243:PRSBER>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHENYLALANINE TRANSFER-RNA; YEAST TRANSFER RNAPHE; ION-INDUCED CLEAVAGES; LEAD BINDING-SITES; COLI TRANSFER-RNA; GROUP-I INTRON; ESCHERICHIA-COLI; PEPTIDYLTRANSFERASE CENTER; CONSERVED NUCLEOTIDES; RESISTANCE MUTATIONS;
Keywords:
antibiotic binding sites; chloramphenicol; erythromycin; metal ion binding sites; peptidyl transferase; rRNA structure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Barta, A Univ Vienna, Vienna Bioctr, Inst Biochem, Dr Bohr Gasse 9-3, A-1030 Vienna, Univ Vienna Dr Bohr Gasse 9-3 Vienna Austria A-1030 1030 Vienna,
Citazione:
S. Dorner e A. Barta, "Probing ribosome structure by europium-induced RNA cleavage", BIOL CHEM, 380(2), 1999, pp. 243-251

Abstract

Divalent metal ions are absolutely required for the structure and catalytic activities of ribosomes, They are partly coordinated to highly structuredRNA, which therefore possesses high-affinity metal ion binding pockets. Asmetal ion induced RNA cleavages are useful for characterising metal ion binding sites and RNA structures, we analysed europium (Eu3+) induced specific cleavages in both 16S and 23S rRNA of E. coli. The cleavage sites were identified by primer extension and compared to those previously identified for calcium, lead, magnesium, and manganese ions, Several Eu3+ cleavage sites, mostly those at which a general metal ion binding site had been already identified, were identical to previously described divalent metal ions. Overall, the Eu3+ cleavages are most similar to the Ca2+ cleavage pattern, probably due to a similar ion radius. Interestingly, several cleavage sites which were specific for Eu3+ were located in regions implicated in the bindingof tRNA and antibiotics. The binding of erythromycin and chloramphenicol, but not tetracycline and streptomycin, significantly reduced Eu3+ cleavage efficiencies in the peptidyl transferase center, The identification of specific Eu3+ binding sites near the active sites on the ribosome will allow touse the fluorescent properties of europium for probing the environment of metal ion binding pockets at the ribosome's active center.

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Documento generato il 03/04/20 alle ore 04:19:02