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Titolo:
An engineered class I transfer RNA with a class II tertiary fold
Autore:
Nissan, TA; Oliphant, B; Perona, JJ;
Indirizzi:
Univ Calif Santa Barbara, Dept Chem, Santa Barbara, CA 93106 USA Univ Calif Santa Barbara Santa Barbara CA USA 93106 Barbara, CA 93106 USA Univra,lif Santa Barbara, Interdept Program Biochem & Mol Biol, Santa Barba Univ Calif Santa Barbara Santa Barbara CA USA 93106 Mol Biol, Santa Barba
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 3, volume: 5, anno: 1999,
pagine: 434 - 445
SICI:
1355-8382(199903)5:3<434:AECITR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMINYL-TRANSFER-RNA; PHENYLALANINE TRANSFER-RNA; ESCHERICHIA-COLI; CRYSTAL-STRUCTURE; FUNCTIONAL CONNECTIVITY; IDENTITY DETERMINANTS; ACCEPTOR IDENTITY; TRANSFER RNA(SER); SYNTHETASE; RECOGNITION;
Keywords:
aminoacyl-tRNA synthetases; extra arm; RNA engineering; RNA structure; tRNA identity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Perona, JJ Univ Calif Santa Barbara, Dept Chem, Santa Barbara, CA 93106 USA Univ Calif Santa Barbara Santa Barbara CA USA 93106 93106 USA
Citazione:
T.A. Nissan et al., "An engineered class I transfer RNA with a class II tertiary fold", RNA, 5(3), 1999, pp. 434-445

Abstract

Structure-based engineering of the tertiary fold of Escherichia coli tRNA(2)(Gln) has enabled conversion of this transfer RNA to a class II structurewhile retaining recognition properties of a class I glutamine tRNA. The new tRNA possesses the 20-nt variable stem-loop of Thermus thermophilus tRNA(Ser). Enlargement of the D-loop appears essential to maintaining a stable tertiary structure in this species, while rearrangement of a base triple in the augmented D-stem is critical for efficient glutaminylation. These data provide new insight into structural determinants distinguishing the class Iand class II tRNA folds, and demonstrate a marked sensitivity of glutaminyl-tRNA synthetase to alteration of tRNA tertiary structure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:27:04