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Titolo:
Changes in expression of neuronal and glial glutamate transporters in rat hippocampus following kainate-induced seizure activity
Autore:
Simantov, R; Crispino, M; Hoc, W; Broutman, G; Tocco, G; Rothstein, JD; Baudry, M;
Indirizzi:
Univ So Calif, Neurosci Program, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 Program, Los Angeles, CA 90089 USA Univ Naples Federico II, Dept Gen & Environm Physiol, Naples, Italy Univ Naples Federico II Naples Italy & Environm Physiol, Naples, Italy Johns Hopkins Univ, Sch Med, Dept Neurol & Neurosci, Baltimore, MD USA Johns Hopkins Univ Baltimore MD USA Neurol & Neurosci, Baltimore, MD USA
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 1, volume: 65, anno: 1999,
pagine: 112 - 123
SICI:
0169-328X(19990219)65:1<112:CIEONA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRAIN DAMAGE SYNDROME; ACID-INDUCED SEIZURES; KAINIC ACID; ASPARTATE TRANSPORTER; OXIDATIVE STRESS; NERVOUS-SYSTEM; MESSENGER-RNA; NEUROTOXICITY; ASTROCYTES; CULTURES;
Keywords:
neuronal plasticity; cell death; transporter; excitatory amino acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Baudry, M Univ So Calif, Neurosci Program, Hedco Neurosci Bldg,Room 309, Los Angeles, Univ So Calif Hedco Neurosci Bldg,Room 309 Los Angeles CA USA 90089
Citazione:
R. Simantov et al., "Changes in expression of neuronal and glial glutamate transporters in rat hippocampus following kainate-induced seizure activity", MOL BRAIN R, 65(1), 1999, pp. 112-123

Abstract

The expression of excitatory amino acid transporters (EAATs) in rat hippocampus was studied following kainic acid-induced seizure activity in vivo and in hippocampal slice cultures. Protein and mRNA levels of the glial (EAAT2) and neuronal (EAAT3) transporters were determined with affinity-purifiedantibodies and oligonucleotide probes, respectively. Kainate treatment decreased EAAT3 immunoreactivity in stratum lacunosum moleculare within 4 h ofseizure onset. Upon pyramidal cell death (5 days after kainate treatment),EAAT3 immunoreactivity in stratum pyramidale of CAI and in stratum lacunosum moleculare was almost completely eliminated. The rapid effect of kainateon EAAT3 expression was confirmed by in situ hybridization; EAAT3 mRNA levels were decreased in CA1 and CA3 regions within 4-8 h of seizure onset. Kainate treatment had an opposite effect on levels and expression of EAAT2. Developmental studies indicated that the rapid regulation of transporter expression was not observed in rats younger than 21 days, an observation congruent with previous reports regarding the resistance of young rats to kainate. In hippocampal organotypic cultures, which lack a major excitatory inputfrom the entorhinal cortex, kainate produced a slow decrease in [H-3]D-aspartate uptake. This study indicates that an early effect of kainate treatment consists of down-regulation of the neuronal transporter EAAT3 in restricted hippocampal regions, together with a modest increase in the expression of the glial transporter EAAT2. Differential regulation of neuronal and glial glutamate transporters may thus play a role in kainate-induced seizure, neurotoxicity and neuronal plasticity. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 26/01/21 alle ore 04:24:56