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Titolo:
Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development
Autore:
Paratcha, GC; Ibarra, GR; Wolansky, MJ; Rodriguez, JA; Azcurra, JM;
Indirizzi:
Univenosnos Aires, Fac Ciencias Exactas & Nat, Lab Biol Celular, RA-1428 Bu Univ Buenos Aires Buenos Aires DF Argentina RA-1428 l Celular, RA-1428 Bu
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 1, volume: 65, anno: 1999,
pagine: 34 - 43
SICI:
0169-328X(19990219)65:1<34:DGPISS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; MUSCARINIC ACETYLCHOLINE-RECEPTOR; LONG-TERM POTENTIATION; RAT STRIATUM; POSTNATAL-DEVELOPMENT; SEROTONIN METABOLISM; DOPAMINE RELEASE; BASAL GANGLIA; B-50 GAP-43; PLASTICITY;
Keywords:
GAP-43/B-50; PKC; SPM; circling training; development; rat striatum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Azcurra, JM CF Melo 4127, RA-1602 Buenos Aires, DF, Argentina CF Melo 4127 Buenos Aires DF Argentina RA-1602 DF, Argentina
Citazione:
G.C. Paratcha et al., "Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development", MOL BRAIN R, 65(1), 1999, pp. 34-43

Abstract

We evaluated the in vitro phosphorylation of the presynaptic substrate of protein kinase C (PKC). GAP-43/B-50 and the PKC activity in the striatum ofrats submitted to a circling training (CT) test during postnatal development. Motor activity at 30 days of age, but not at other ages. produced a unilateral reduction (-29.5%: p < 0.001) in the level of GAP-43/B-50 endogenous phosphorylation in the contralateral striatum with respect to the ipsilateral side, while non-trained control animals did not show asymmetric differences. Compared to controls, the contralateral striatum of trained animals also showed a significant reduction (-29.3%; p < 0.001) in the incorporation of P-32-phosphate into GAP-43. This decreased in vitro GAP-43 phosphorylation was seen at 30 min, but not immediately after circling motor behavior. This contralateral change in GAP-43 phosphorylation correlated with the running speed developed by the animals [(r = 0.9443, p = 0.0046, n = 6, relative to control group) and (r = 0.8813, p = 0.0203, n = 6, with respect to the ipsilateral side of the exercised animals)]. On the contrary. GAP-43/B-50 irnmunoblots did not show changes in the amount of this phosphoprotein among the different experimental groups. Back phosphorylation assays, performed in the presence of bovine purified PKC, increased the level of GAP-43/B-50 phosphorylation in the striatum contralateral to the sense of turning [(22%; p < 0.05, with respect to ipsilateral side of the same trained group)and (+21%; p < 0.05, relative to control group)]. Taken together, these results demonstrate that the activity developed in the CT test induces a reduction in the phosphorylation state of GAP43/B-50 in the specific site for PKC. We conclude that general markers of activity-dependent neuronal plasticity are also altered in the same period that long-lasting changes in striatal neuroreceptors are triggered by circling motor behavior. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 12:11:49