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Titolo:
Postsynaptic expression of Ca2+-permeable AMPA-type glutamate receptor channels by viral-mediated gene transfer
Autore:
Sudo, M; Okado, H; Iino, M; Tsuzuki, K; Miwa, A; Kanegae, Y; Saito, I; Ozawa, S;
Indirizzi:
Gunma Univ, Sch Med, Dept Physiol, Maebashi, Gumma 3718511, Japan Gunma Univ Maebashi Gumma Japan 3718511 l, Maebashi, Gumma 3718511, Japan Tokyoanetropolitan Inst Neurosci, Dept Neurobiol, Fuchu, Tokyo 1838526, Jap Tokyo Metropolitan Inst Neurosci Fuchu Tokyo Japan 1838526 o 1838526, Jap Japan Sci & Technol Corp, CREST, Kawaguchi, Saitama 3320012, Japan Japan Sci & Technol Corp Kawaguchi Saitama Japan 3320012 a 3320012, Japan Univ Tokyo, Inst Med Sci, Genet Mol Lab, Tokyo 1080071, Japan Univ Tokyo Tokyo Japan 1080071 Sci, Genet Mol Lab, Tokyo 1080071, Japan
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 2, volume: 65, anno: 1999,
pagine: 176 - 185
SICI:
0169-328X(19990305)65:2<176:PEOCAG>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT HIPPOCAMPAL-NEURONS; CENTRAL NERVOUS-SYSTEM; AMINO-ACID RECEPTORS; CA2+ PERMEABILITY; FUNCTIONAL EXPRESSION; NEOCORTICAL NEURONS; SUBUNIT COMPOSITION; SPIDER TOXIN; ION FLOW; CELLS;
Keywords:
adenovirus; gene transfer; Ca2+-permeable AMPA receptor; PC12 cell; hippocampal neuron; glutamatergic synapse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Ozawa, S Gunma8511,, Sch Med, Dept Physiol, 3-39-22 Showa Machi, Maebashi,Gumma 371 Gunma Univ 3-39-22 Showa Machi Maebashi Gumma Japan 3718511 a 371
Citazione:
M. Sudo et al., "Postsynaptic expression of Ca2+-permeable AMPA-type glutamate receptor channels by viral-mediated gene transfer", MOL BRAIN R, 65(2), 1999, pp. 176-185

Abstract

The ability to artificially express a particular receptor protein in the postsynaptic sites of neurons in the central nervous system (CNS) would be useful for the study of synaptic function of cloned receptor genes as well as for gene therapy of neurological disorders caused by dysfunction of postsynaptic receptors. In this study, we aimed to express the cDNA of unedited GluR2, subunit of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptor that forms inwardly rectifying and Ca2+-permeable channel in CNS neurons by using adenoviral-mediated gene transfer. For this purpose, we have constructed a recombinant adenovirus bearing anexpression-switching unit, where the unedited GluR2 cDNA can be activated by the Cre recombinase-mediated excisional deletion of a stuffer DNA interposed between the promotor and the coding region. When PC12 cells were infected with this recombinant adenovirus together with an adenovirus expressingCre recombinase, the inwardly rectifying and Ca2+-permeable AMPA receptor channels were expressed in nearly 100% of infected cells. Two days after co-infection of cultured rat hippocampal neurons with these adenoviruses, fast excitatory neurotransmission in the glutamatergic synapse was mediated predominantly by the inwardly rectifying and Ca2+-permeable AMPA receptor channels. This indicates that the native AMPA receptors in the postsynaptic sites of the glutamatergic synapse are replaced rapidly with recombinant receptors newly produced by the viral-mediated gene transfer. (C) 1999 ElsevierScience B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:16:18