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Titolo:
Lysozyme net charge and ion binding in concentrated aqueous electrolyte solutions
Autore:
Kuehner, DE; Engmann, J; Fergg, F; Wernick, M; Blanch, HW; Prausnitz, JM;
Indirizzi:
Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 em Engn, Berkeley, CA 94720 USA Lawrence Berkeley Lab, Div Chem Sci, Berkeley, CA 94720 USA Lawrence Berkeley Lab Berkeley CA USA 94720 m Sci, Berkeley, CA 94720 USA
Titolo Testata:
JOURNAL OF PHYSICAL CHEMISTRY B
fascicolo: 8, volume: 103, anno: 1999,
pagine: 1368 - 1374
SICI:
1520-6106(19990225)103:8<1368:LNCAIB>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHENOMENOLOGICAL LINKAGE RELATIONS; CONTINUUM ELECTROSTATIC MODEL; INDUCED PROTEIN PRECIPITATION; DYNAMIC LIGHT-SCATTERING; INTERFACIAL THERMODYNAMICS; GLOBULAR-PROTEINS; TITRATION CURVES; PHASE-EQUILIBRIA; ACIDIC RESIDUES; PK(A);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Prausnitz, JM Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 ley, CA 94720 USA
Citazione:
D.E. Kuehner et al., "Lysozyme net charge and ion binding in concentrated aqueous electrolyte solutions", J PHYS CH B, 103(8), 1999, pp. 1368-1374

Abstract

Hydrogen-ion titrations were conducted for hen-egg-white lysozyme in solutions of potassium chloride over the range pH 2.5-11.5 and for ionic strengths to 2.0 M. The dependence of lysozyme's net proton charge, z(P), on pH and ionic strength in potassium chloride solution is measured. From the ionic-strength dependence of z(P), interactions of lysozyme with potassium and chloride ions are calculated using the molecular-thermodynamic theory of Fraaije and Lyklema.(1) Lysozyme interacts preferentially with up to 12 chloride ions at pH 2.5. The observed dependence of ion-protein interactions on pH and ionic strength is explained in terms of electric-double-layer theory. New experimental pK(a) data are reported for 11 amino acids in potassium chloride solutions of ionic strength to 3.0 M.

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Documento generato il 20/01/20 alle ore 16:03:15