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Titolo:
Amplification of the antibody response by C3b complexed to antigen throughan ester link
Autore:
Villiers, MB; Villiers, CL; Laharie, AM; Marche, PN;
Indirizzi:
Univ-38054ble 1, CEA,Lab Immunochim, Dept Biol Mol & Struct, INSERM,U238, F Univ Grenoble 1 Grenoble France 9 Dept Biol Mol & Struct, INSERM,U238, F
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 6, volume: 162, anno: 1999,
pagine: 3647 - 3652
SICI:
0022-1767(19990315)162:6<3647:AOTARB>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION COMPLEX; HUMORAL IMMUNE-RESPONSE; T-DEPENDENT ANTIGEN; B-CELL RESPONSE; LYMPHOCYTES-B; TETANUS TOXIN; NATURAL ANTIBODY; RECEPTOR; ADJUVANT; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Villiers, MB Univ7Grenoble 1, CEA,Lab Immunochim, Dept Biol Mol & Struct, INSERM,U238, 1 Univ Grenoble 1 17 Rue Martyrs Grenoble France 9 ERM,U238, 1
Citazione:
M.B. Villiers et al., "Amplification of the antibody response by C3b complexed to antigen throughan ester link", J IMMUNOL, 162(6), 1999, pp. 3647-3652

Abstract

Complement C3 has been described as playing an important role in the cell-mediated immune response, C3b has the capacity to covalently bind Ag and then to stimulate in vitro Ag presentation to T lymphocytes, To verify this observation in vivo, we prepared and purified covalent human C3b-Ag complexes using lysozyme (HEL) as Ag, The characterization of these HEL-C3b complexes indicates that they are representative of those susceptible to be generated in physiological conditions. Mice were immunized with 0.1 to 0.6 mu g of either free HEL, MEL + C3b, HEL-C3b, or MEL + CFA, Response was assessed after two i.p. injections by quantification of specific Ab production. Immunization with either HEL-C3b complexes or HEL + CFA leads to anti-MEL IgG production whereas free HEL or HEL + C3b was ineffective. Either HEL-C3b or HEL + CFA immunizations led to a similar Ig subclasse patterns, including IgG1, IgG2a, IgA, and IgM,Our experiments provide the first evidence for modulation of specific Ab response by C3b when it is bound to Ag through a physiological-like link, Taken together with previous data concerning Ab response following recombinant HEL-C3d immunization, cellular events such as processing of C3b-Ag by APCand recognition by T lymphocytes, this present result underlines the importance of C3b and its fragments in stimulation of the immune system, throughthe multiplicity and complementarity of its interactions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:18:20