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Titolo:
IL-2-dependent expression of genes involved in cytoskeleton organization, oncogene regulation, and transcriptional control
Autore:
Herblot, S; Chastagner, P; Samady, L; Moreau, JL; Demaison, C; Froussard, P; Liu, XY; Bonnet, J; Theze, J;
Indirizzi:
Inst Pasteur, Unite Immunogenet Cellulaire, F-75724 Paris 15, France Inst Pasteur Paris France 15 ogenet Cellulaire, F-75724 Paris 15, France Univ Bordeaux 2, Lab Immunol Mol, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 ol Mol, F-33076 Bordeaux, France Acad Sinica, Shanghai Inst Biochem, Shanghai 200031, Peoples R China Acad Sinica Shanghai Peoples R China 200031 ghai 200031, Peoples R China
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 6, volume: 162, anno: 1999,
pagine: 3280 - 3288
SICI:
0022-1767(19990315)162:6<3280:IEOGII>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELEMENT-BINDING-PROTEIN; RECEPTOR-ALPHA GENE; T-CELL LINES; IL-2 RECEPTOR; INTERLEUKIN-2 RECEPTOR; BETA-CATENIN; C-MYC; CHAIN EXPRESSION; ZINC-FINGERS; PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Theze, J Instrissteur, Unite Immunogenet Cellulaire, 25 Rue Docteur Roux, F-75724 Pa Inst Pasteur 25 Rue Docteur Roux Paris France 15 oux, F-75724 Pa
Citazione:
S. Herblot et al., "IL-2-dependent expression of genes involved in cytoskeleton organization, oncogene regulation, and transcriptional control", J IMMUNOL, 162(6), 1999, pp. 3280-3288

Abstract

IL-2 induces growth, differentiation, and/or apoptosis of lymphoid cells. To study further the molecular basis of IL-2 function; we used a cDNA subtraction approach involving a cell line grown in IL-2 or IL-4. From the corresponding library, 66 nonredundant sequences were characterized; 16 of them encode identified proteins. The kinetics of in vitro expression of 8 selected sequences, the functions of which could he associated with IL-2-induced T cell activation/differentiation, was investigated using an IL-2-dependentT cell lint. IL-2 increased the expression of cytoskeleton proteins (cy-tubulin), oncogene-regulating proteins (CCCTC-binding factor, Jun inhibitor factor-1), and transcription factors (E2F-4, cyclic AMP-responsive element-binding protein, zhx-1), IL-2 also regulated the expression of genes coding for multifunctional proteins, e.g., beta-catenin and nucleolin, These results were verified using Con A-induced T cell blasts stimulated or not by IL-2. The in vivo expression of four of these genes was also analyzed in spleen and lymph node cells of IL-2-deficient and MRL/lpr mice, which both have high numbers of activated cells, but the latter have intact IL-2 expression. The expression of beta-catenin, CCCTC-binding factor, Jun inhibitor factor-1, and nucleolin was significantly higher in MRL/lpr animals. A similar analysis of thymocytes from IL-2(-/-) and IL-2(+/-) mice demonstrated the same expression patterns of the 4 sequences in these strains. The expression of the IL-2-induced genes described herein is similar to the regulatory pattern of IL-2R alpha. Taken together, our data provide additional evidence for the pleiotropic action of IL-2 in the periphery and IL-2 independence ofmolecular processes involved in thymocyte differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:09:00