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Titolo:
Breeding of flocculent industrial alcohol yeast strains by self-cloning ofthe flocculation gene FLO1 and repeated-batch fermentation by transformants
Autore:
Ishida-Fujii, K; Goto, S; Sugiyama, H; Takagi, Y; Saiki, T; Takagi, M;
Indirizzi:
NEDO, R&D Ctr, Alcohol Prod Head Off, Inage Ku, Chiba 2630031, Japan NEDOChiba Japan 2630031 l Prod Head Off, Inage Ku, Chiba 2630031, Japan Sapporo Breweries Ltd, Brewing Res Labs, Yaizu 4250013, Japan Sapporo Breweries Ltd Yaizu Japan 4250013 Res Labs, Yaizu 4250013, Japan Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 otechnol, Bunkyo Ku, Tokyo 1138657, Japan
Titolo Testata:
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
fascicolo: 5, volume: 44, anno: 1998,
pagine: 347 - 353
SICI:
0022-1260(199810)44:5<347:BOFIAY>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; BREWERS-YEAST; CONSTRUCTION; DISRUPTION; SEQUENCE;
Keywords:
alcohol yeast; flocculation; FLO1; industrial alcohol production; polyploidy; repeated-batch fermentation; Saccharomyces cerevisiae; self-cloning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Ishida-Fujii, K NEDO,630031,r, Alcohol Prod Head Off, Inage Ku, 4-5-1 Inagehigashi, Chiba 2 NEDO 4-5-1 Inagehigashi Chiba Japan 2630031 ashi, Chiba 2
Citazione:
K. Ishida-Fujii et al., "Breeding of flocculent industrial alcohol yeast strains by self-cloning ofthe flocculation gene FLO1 and repeated-batch fermentation by transformants", J GEN A MIC, 44(5), 1998, pp. 347-353

Abstract

A nonflocculent industrial polyploid yeast strain, Saccharomyces cerevisiae 396-9-6V, was converted to a flocculent one by introducing a functional FLO1 gene at the URA3 locus. The flocculent strain FSC27 obtained was a so-called self-cloned strain, having no bacterial DNA. FSC27 cells could be easily recovered for reuse from fermentation mash without any physical energy,The strain produced a concentration of alcohol as high as 396-9-6V, although the fermentation rate of FSC27 was slightly lower than that of 396-9-6V. When uracil was added to the medium or when URA3 was reintroduced into FSC27 (named FSCU-L18), the fermentation rate and the growth rate increased, and the ethanol concentration produced was higher than that produced by the parent strain, The stable flocculation and high ethanol productivity were observed by using FSCU-L18 during 10 cycles of repeated-batch fermentation test.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 20:15:00