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Titolo:
The nucleolar phosphoprotein B23 redistributes in part to the spindle poles during mitosis
Autore:
Zatsepina, OV; Rousselet, A; Chan, PK; Olson, MOJ; Jordan, EG; Bornens, M;
Indirizzi:
Inst Curie, CNRS, UMR 144, F-75248 Paris, France Inst Curie Paris FranceF-75248 ie, CNRS, UMR 144, F-75248 Paris, France Univ London Kings Coll, Randall Inst, London WC2B 5RL, England Univ LondonKings Coll London England WC2B 5RL London WC2B 5RL, England Baylor Coll Med, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ylor Coll Med, Houston, TX 77030 USA Univ Mississippi, Med Ctr, Jackson, MS 39216 USA Univ Mississippi JacksonMS USA 39216 ppi, Med Ctr, Jackson, MS 39216 USA Moscow MV Lomonosov State Univ, AN Belozersky Inst Phys & Chem Biol, Moscow Moscow MV Lomonosov State Univ Moscow Russia 119899 & Chem Biol, Moscow
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 4, volume: 112, anno: 1999,
pagine: 455 - 466
SICI:
0021-9533(199902)112:4<455:TNPBRI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOTIC APPARATUS PROTEIN; AMINO-ACID-SEQUENCE; PRENUCLEOLAR BODIES; NUCLEAR MATRIX; CELL-CYCLE; PHOSPHORYLATION SITE; CHROMOSOME PERIPHERY; LOCALIZATION SIGNALS; HELA-CELLS; 2 FORMS;
Keywords:
mitosis; mitotic spindle; nucleolus; B23; NuMA; taxol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Bornens, M Inst Curie, CNRS, UMR 144, F-75248 Paris, France Inst Curie Paris France F-75248 MR 144, F-75248 Paris, France
Citazione:
O.V. Zatsepina et al., "The nucleolar phosphoprotein B23 redistributes in part to the spindle poles during mitosis", J CELL SCI, 112(4), 1999, pp. 455-466

Abstract

B23 is a major phosphoprotein in the interphasic nucleolus where it is involved in the assembly of pre-ribosomes. Using several cultured animal cells, we report that, in addition to the known redistribution of the protein during mitosis, B23 also becomes associated with mitotic spindle poles starting from early prometaphase onwards. Colocalization of B23 with the protein NuMA (Nuclear Mitotic Apparatus protein) was studied in mitotic cells and taxol-arrested cells, During the onset of mitosis, we observed that a fraction of B23 associates with, and dissociates from, the poles later than NuMA,At metaphase, both proteins are colocalized at the poles. The polar redistribution of both B23 and NuMA is mediated by microtubules. In taxol-treatedcells, B23 is associated with the microtubule minus ends in the center of mitotic asters together with NuMA, Association of B23 with microtubule minus ends of mitotic asters was further confirmed with an in vitro assay, where B23 was found by western blotting to co-sediment with taxol-induced microtubule asters formed in a mitotic cell extract. Immunolabeling demonstratedthat B23 and NuMA were both present at the center of the asters, Furthermore, an additional hyperphosphorylated form of B23 appeared when microtubuleasters formed and associated with the asters, Immunodepletion of B23 from the mitotic extract revealed that taxol-induced microtubule asters were still observed in B23-immunodepleted mitotic extract, indicating that the presence of B23 at the poles is unlikely to be essential for spindle formation or stabilisation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 10:38:41