Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CONSTITUTIVE AND VARIABLE REGIONS OF Z-DISK TITIN CONNECTIN IN MYOFIBRIL FORMATION - A DOMINANT-NEGATIVE SCREEN/
Autore:
PECKHAM M; YOUNG P; GAUTEL M;
Indirizzi:
EUROPEAN MOL BIOL LAB,STRUCT BIOL DIV,POSTFACH 102209 D-69012 HEIDELBERG GERMANY EUROPEAN MOL BIOL LAB,STRUCT BIOL DIV D-69012 HEIDELBERG GERMANY UNIV LONDON KINGS COLL,RANDALL INST LONDON WC2R 2LS ENGLAND
Titolo Testata:
Cell structure and function
fascicolo: 1, volume: 22, anno: 1997,
pagine: 95 - 101
SICI:
0386-7196(1997)22:1<95:CAVROZ>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SKELETAL-MUSCLE; Z-LINE; IMMUNOELECTRON MICROSCOPY; MONOCLONAL-ANTIBODIES; CONNECTIN FILAMENTS; SARCOMERE MATRIX; ELASTIC PROTEIN; TITIN; MYOSIN; PURIFICATION;
Keywords:
TITIN; CONNECTIN; Z-DISK; SARCOMERE ASSEMBLY; ISOFORMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
M. Peckham et al., "CONSTITUTIVE AND VARIABLE REGIONS OF Z-DISK TITIN CONNECTIN IN MYOFIBRIL FORMATION - A DOMINANT-NEGATIVE SCREEN/", Cell structure and function, 22(1), 1997, pp. 95-101

Abstract

The giant muscle protein titin (connectin) is assumed to play a crucial role in the control of Z-disk assembly. Analysis of the Z-disk region of titin/connectin revealed a novel 45 residue repeat that is spliced in variable copy numbers. The repeat region is coexpressed in normal human myocardium in size variants corresponding to between 5 and 7 repeats. Smaller isoforms can be detected in muscles with thinner Z-disks like M. psoas. Sequence analysis of chicken breast muscle titin/connectin reveals that in this tissue, where Z-disks are thin, only two Z-repeats are expressed. The greatly variable thickness of Z-disks is therefore correlated to a region of variable length in titin/connectin,constructed from a novel protein building block. Transfection experiments in myogenic cell lines demonstrate that overexpression of the entire integral Z-disk region of titin leads to a disruption of sarcomereassembly. The differentially expressed titin/connectin regions, however, show no dominant-negative effects on myofibril assembly and are not targeted to Z-disks. This supports the idea that the Z-repeat regionof titin/connectin is responsible for the control Z-disk thickness asan element of highly variable length, due to extensive differential splicing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:33:53