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Titolo:
Antioxidant BO-653 and human macrophage-mediated LDL oxidation
Autore:
Muller, K; Carpenter, KLH; Freeman, MA; Mitchinson, MJ;
Indirizzi:
UnivdCambridge, Dept Pathol, Div Cellular Pathol, Cambridge CB2 1QP, Englan Univ Cambridge Cambridge England CB2 1QP thol, Cambridge CB2 1QP, Englan
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 1, volume: 30, anno: 1999,
pagine: 59 - 71
SICI:
1071-5762(1999)30:1<59:ABAHML>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HUMAN MONOCYTE-MACROPHAGES; HUMAN ATHEROSCLEROTIC LESIONS; SWEDISH TRIAL PQRST; PROTEIN-KINASE-C; ALPHA-TOCOPHEROL; VITAMIN-E; FEMORAL ATHEROSCLEROSIS; LIPID-PEROXIDATION; SCAVENGER RECEPTOR;
Keywords:
BO-653; low density lipoprotein; oxidation; macrophages (human); antioxidants; atherosclerosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Muller, K UniveCambridge, Dept Pathol, Div Cellular Pathol, Tennis Court Rd, Cambridg Univ Cambridge Tennis Court Rd Cambridge England CB2 1QP mbridg
Citazione:
K. Muller et al., "Antioxidant BO-653 and human macrophage-mediated LDL oxidation", FREE RAD RE, 30(1), 1999, pp. 59-71

Abstract

Oxidation of LDL is now widely accepted to be involved in atherogenesis. The aim of this study was to examine the effect of BO-653, a strong radical scavenger and antioxidant, on oxidation of LDL by human macrophages in vitro. Fifty mu g/ml LDL protein was incubated with macrophages in Ham's F10 medium, supplemented with additional Fe2+, for up to 48 h. Then the medium was analysed by LDL agarose gel electrophoresis, the thiobarbituric acid assay and gas chromatography. In the absence of added exogenous antioxidants, after 24 h LDL oxidation produced 30.48 nmoles MDA equivalents/mg LDL protein and a relative electrophoretic mobility of 4.74. Linoleic acid (18 : 2), arachidonic acid (20 : 4) and cholesterol were depleted and 7 beta-hydroxycholesterol was generated. BO-653 completely inhibited this cell-mediated oxidation of LDL in concentrations as low as 5 mu M, being more effective than either alpha-tocopherol or probucol, which completely inhibited oxidationat 200 and 80 mu M and only partially at 80 and 8 mu M, respectively. Thisinhibition of cell-mediated LDL oxidation was not due to toxicity, as alpha-tocopherol, probucol and BO-653 were not toxic for the macrophages at theconcentrations tested. Eighty mu M alpha-tocopherol, 8 mu M probucol and 5mu M BO-653 significantly reduced the toxicity to the oxidising culture caused by LDL oxidation. The results show that in this system BO-653 is a more effective antioxidant than alpha-tocopherol or probucol.

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Documento generato il 29/02/20 alle ore 02:38:44