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Titolo:
Dexamethasone ameliorates oxidative DNA damage induced by benzene and LPS in mouse bone marrow
Autore:
Tuo, JS; Deng, XS; Loft, S; Poulsen, HE;
Indirizzi:
Rigshosp, Dept Clin Pharmacol Q7642, DK-2200 Copenhagen N, Denmark Rigshosp Copenhagen Denmark N macol Q7642, DK-2200 Copenhagen N, Denmark Univ Copenhagen, Panum Inst, Dept Pharmacol, DK-2200 Copenhagen, Denmark Univ Copenhagen Copenhagen Denmark DK-2200 , DK-2200 Copenhagen, Denmark
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 1, volume: 30, anno: 1999,
pagine: 29 - 36
SICI:
1071-5762(1999)30:1<29:DAODDI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; POLYUNSATURATED FATTY-ACIDS; GLUCOCORTICOIDS INHIBIT; SUPEROXIDE ANION; INHALED BENZENE; MICE; CELLS; RAT; INDUCTION; MACROPHAGES;
Keywords:
benzene; lipopolysaccharide (LPS); dexamethasone (DEX); 8-hydroxy-2 '-deoxyguanosine (8-oxodG); single cell gel electrophoresis (Comet assay); reactive nitrogen species (RNS);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Poulsen, HE Rigshosp,nmark Clin Pharmacol Q7642, 20 Tagensvej, DK-2200 Copenhagen N, De Rigshosp 20 Tagensvej Copenhagen Denmark N 0 Copenhagen N, De
Citazione:
J.S. Tuo et al., "Dexamethasone ameliorates oxidative DNA damage induced by benzene and LPS in mouse bone marrow", FREE RAD RE, 30(1), 1999, pp. 29-36

Abstract

Mice were grouped to receive vehicle, dexamethasone (DEX), lipopolysaccharide (LPS), benzene (BZ, 200 mg/kg) and combinations: LPS + DEX, BZ + DEX, LPS + BZ, LPS + DEX + BZ. The DNA damage in bone marrow cells from BZ group was enhanced 2.8-fold measured by nuclear 8-hydroxy-2'-deoxyguanosine (8-oxodG) and 1.4-fold measured by Comet score (index of DNA breaks) (p < 0.05). In the BZ + DEX group, 8-oxodG level and the Comet score were lowered to 65% and 76% respectively of that in the BZ group (p < 0.05). The BZ + LPS caused a 3.9-fold increase in 8-oxodG and a 1.6-fold increase in the Comet score (p < 0.05). The LPS + DEX + BZ lowered 8-oxodG level and the Comet score to 50% and 78% of the values in the LPS + BZ group, respectively tp < 0.05). Nitrate/nitrite levels in serum were higher after BZ + LPS treatment than after all other treatments. Both 8-oxodG level and the Comet scores werecorrelated to the serum nitrate/nitrite level across all the treatments (r= 0.55, p < 0.01 and r = 0.69, p < 0.01, respectively). In bone marrow cells the 8-oxodG correlated with the Comet scores (r = 0.80, p < 0.01). We conclude that DEX administration can reduce the DNA damage from BZ treatment and from the combination of BZ and LPS. The correlation of DNA damage with nitrate/nitrite indicates the possible involvement of reactive nitrogen species (RNS) in the interaction between BZ and the inflammatory reaction stimulated by LPS. The 8-oxodG determination is more sensitive than strand break analysis by the Comet assay in bone marrow in vivo in mice for measuring the BZ-induced DNA damage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 21:48:21