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Titolo:
Glutathione (GSH) and the toxicity of oxidised low-density lipoprotein to human monocyte-macrophages
Autore:
Hardwick, SJ; Carpenter, KLH; Allen, EA; Mitchinson, MJ;
Indirizzi:
UnivdCambridge, Dept Pathol, Div Cellular Pathol, Cambridge CB2 1QP, Englan Univ Cambridge Cambridge England CB2 1QP thol, Cambridge CB2 1QP, Englan
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 1, volume: 30, anno: 1999,
pagine: 11 - 19
SICI:
1071-5762(1999)30:1<11:G(ATTO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; ATHEROSCLEROTIC LESIONS; CYTO-TOXICITY; VITAMIN-E; CYTOTOXICITY; OXYSTEROLS; ALDEHYDES; GENERATION; INHIBITION; OXIDATION;
Keywords:
GSH; toxicity; 4-hydroxynonenal; oxidised LDL; monocyte-macrophages (human); atherosclerosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Hardwick, SJ UniveCambridge, Dept Pathol, Div Cellular Pathol, Tennis Court Rd, Cambridg Univ Cambridge Tennis Court Rd Cambridge England CB2 1QP idg
Citazione:
S.J. Hardwick et al., "Glutathione (GSH) and the toxicity of oxidised low-density lipoprotein to human monocyte-macrophages", FREE RAD RE, 30(1), 1999, pp. 11-19

Abstract

Macrophage death, believed to be an important event in the pathogenesis ofhuman atherosclerosis, can be induced by oxidised low-density lipoprotein (LDL) in vitro. Supplementation of the culture medium with 5 mM GSH significantly protected human monocyte-macrophages in vitro against the toxicity of copper-oxidised LDL. Oxidation products of LDL include the aldehyde 4-hydroxynonenal (HNE). We present evidence that conjugation of HNE by GSH contributes to this protection. In the absence of cells, HPLC analysis showed there were marked reductions in the levels of both pure HNE and HNE in copper-oxidised LDL in the presence of GSH. However, GSH did not reverse protein modification, as judged by agarose gel electrophoresis, nor did it influence the depletion of polyunsaturated fatty acids, which were assessed using gas chromatography. Thepossible implications for human atherosclerosis are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 07:47:11