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Titolo:
Expression of homing-associated cell adhesion molecule (H-CAM/CD44) on human CD34(+) hematopoietic progenitor cells
Autore:
Deguchi, T; Komada, Y; Sugiyama, K; Zhang, XL; Azuma, E; Yamamoto, H; Sakurai, M;
Indirizzi:
Mie Univ, Sch Med, Dept Pediat, Tsu, Mie 5148507, Japan Mie Univ Tsu Mie Japan 5148507 Med, Dept Pediat, Tsu, Mie 5148507, Japan Mie Univ, Sch Med, Dept Clin Immunol, Tsu, Mie 514, Japan Mie Univ Tsu Mie Japan 514 ch Med, Dept Clin Immunol, Tsu, Mie 514, Japan Natl Mie Chuo Hosp, Dept Pediat, Hisai, Mie, Japan Natl Mie Chuo Hosp Hisai Mie Japan Hosp, Dept Pediat, Hisai, Mie, Japan
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 3, volume: 27, anno: 1999,
pagine: 542 - 552
SICI:
0301-472X(199903)27:3<542:EOHCAM>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEM-CELLS; PERIPHERAL-BLOOD; MARROW CULTURES; IN-VITRO; APOPTOSIS; DIFFERENTIATION; CD44; ANTIBODIES; RECEPTOR; PROLIFERATION;
Keywords:
CD44; hematopoiesis; apoptosis; hematopoietic progenitor cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Komada, Y Mie Univ, Sch Med, Dept Pediat, 2-174 Edobashi, Tsu, Mie 5148507, Japan Mie Univ 2-174 Edobashi Tsu Mie Japan 5148507 Mie 5148507, Japan
Citazione:
T. Deguchi et al., "Expression of homing-associated cell adhesion molecule (H-CAM/CD44) on human CD34(+) hematopoietic progenitor cells", EXP HEMATOL, 27(3), 1999, pp. 542-552

Abstract

We investigated the expression of CD44 molecule on CD34(+) hematopoietic progenitor cells. Significantly lower expression of CD44 was observed on bone marrow (BM) CD34(+) cells compared with circulating CD34+ cells in cord blood and peripheral blood. Using fluorescence-activated cell sorting, humanCD34(+) BM cells were fractionated into CD44(+) and CD44(-) populations. Immunofluorescence analysis revealed that the majority of CD34(+)CD44(-) cells expressed B-lymphocyte-associated CD10 and CD19 antigens, whereas only apart of CD34(+) CD44(+) cells were positive for CD19, Myeloid and erythroid progenitor cells were found predominantly in CD34(+) CD44(+) cell fractions. In short-term suspension cultures, cell proliferation and G(1) --> S transition in the cell cycle were enhanced in CD34(+)CD44(+) cells. In contrast, a large part of CD34(+)CD44(-) cells underwent apoptotic cell death. Although co-culture with BM stromal cells could partially prevent CD34(+)CD44(-) cells from undergoing apoptosis, significant increase of apoptotic cells was consistently observed. Furthermore, CD34(+)CD44(-) cells plated on BMstromal cells could differentiate into CD34(-)CD44(-)CD10(-)CD19(+) cells. These findings suggest that CD34+CD44- cells expressing CD19 would represent unique B-lymphocyte-committed precursors in BM, which might undergo apoptotic cell death in the early steps of B-cell differentiation. (C) 1999 International Society for Experimental Hematology. Published by Elsevier Science Inc.

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Documento generato il 05/04/20 alle ore 08:47:26