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Titolo:
Establishment of persistent infection with HIV-1 abrogates the caspase-3-dependent apoptotic signaling pathway in U937 cells
Autore:
Tanaka, Y; Kameoka, M; Ota, K; Itaya, A; Ikuta, K; Yoshihara, K;
Indirizzi:
Nara Med Univ, Dept Biochem, Nara 6348521, Japan Nara Med Univ Nara Japan 6348521 Univ, Dept Biochem, Nara 6348521, Japan Hokkaido,Univ, Inst Immunol Sci, Sect Serol, Kita Ku, Sapporo, Hokkaido 060 Hokkaido Univ Sapporo Hokkaido Japan 060 , Kita Ku, Sapporo, Hokkaido 060
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 2, volume: 247, anno: 1999,
pagine: 514 - 524
SICI:
0014-4827(19990315)247:2<514:EOPIWH>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; ICE-FAMILY; CYSTEINE PROTEASES; T-CELLS; CPP32-LIKE ACTIVITY; ICE/CED-3 PROTEASE; MEDIATED APOPTOSIS; DEATH HYPOTHESIS; HL-60 CELLS; INHIBITION;
Keywords:
HIV-1; U937; caspase-3; teniposide; camptothecin; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, Y Nara Med Univ, Dept Biochem, Shijo Cho 840, Nara 6348521, Japan Nara Med Univ Shijo Cho 840 Nara Japan 6348521 a 6348521, Japan
Citazione:
Y. Tanaka et al., "Establishment of persistent infection with HIV-1 abrogates the caspase-3-dependent apoptotic signaling pathway in U937 cells", EXP CELL RE, 247(2), 1999, pp. 514-524

Abstract

Treatment of 26L cells, a subclone obtained from U937 cells, with TNF-alpha or DNA-damaging agents such as teniposide (VM26) and camptothecin (CPT) induced morphologically and biochemically typical apoptotic changes, including the activation of procaspase-3. The cells persistently infected with HIV-1 (26L/HIV), however, showed a marked resistance to VM26 and CPT, whereas they hardly lost the sensitivity to TNF-alpha. TNF-alpha-induced apoptosis of 26L/HIV cells proceeded without the increase in caspase-3 activity, indicating that signaling for apoptosis in the infected cells proceeded throughan alternative caspase-3-independent pathway which could respond to TNF-alpha but not to VM26 and CPT. The evidence that p-toluenesulfonyl-L-lysine chloromethyl ketone (a trypsin-like serine protease inhibitor) blocked VM26-and CPT-induced apoptotic changes but not TNF-alpha-induced apoptosis alsosupported the existence of the alternative TNF-alpha-inducible pathway. The results also suggest that a TLCK-sensitive protease is involved upstream of the procaspase-3 activation process and that the protease is essential for the progress of VM26- and CPT-induced apoptosis. The similar effect of HIV-1-productive infection on the apoptosis induced by the DNA-damaging agents was also confirmed by utilizing U1 cells, which are latently HIV-1-infected U937 cells. The cells became resistant to these agents after induction of the viral production by pretreatment with PMA. These results suggest that persistent HIV-1 infection blocks an apoptotic pathway triggered by DNA damaging agents through the inhibition of the procaspase-3 activation process. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:50:16