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Titolo:
In vivo expression of the nucleolar group I intron-encoded I-DirI homing endonuclease involves the removal of a spliceosomal intron
Autore:
Vader, A; Nielsen, H; Johansen, S;
Indirizzi:
Univ Tromso, Dept Mol Cell Biol, Inst Med Biol, N-9037 Tromso, Norway UnivTromso Tromso Norway N-9037 l, Inst Med Biol, N-9037 Tromso, Norway Univ,Copenhagen, Panum Inst, Dept Med Biochem & Genet, DK-2200 Copenhagen N Univ Copenhagen Copenhagen Denmark N ochem & Genet, DK-2200 Copenhagen N
Titolo Testata:
EMBO JOURNAL
fascicolo: 4, volume: 18, anno: 1999,
pagine: 1003 - 1013
SICI:
0261-4189(19990215)18:4<1003:IVEOTN>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-I; NUCLEAR RIBOSOMAL DNA; FUNCTIONAL MESSENGER-RNA; PROTEIN-CODING GENES; PHYSARUM-POLYCEPHALUM; SACCHAROMYCES-CEREVISIAE; TRANSLATION INITIATION; INTERVENING SEQUENCE; NUCLEOTIDE-SEQUENCE; TRYPANOSOMA-BRUCEI;
Keywords:
Didymium iridis; group I intron; homing endonuclease; mRNA processing; ribozyme;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Vader, A Univ Tromso, Dept Mol Cell Biol, Inst Med Biol, N-9037 Tromso, Norway Univ Tromso Tromso Norway N-9037 ed Biol, N-9037 Tromso, Norway
Citazione:
A. Vader et al., "In vivo expression of the nucleolar group I intron-encoded I-DirI homing endonuclease involves the removal of a spliceosomal intron", EMBO J, 18(4), 1999, pp. 1003-1013

Abstract

The Didymium iridis DiSSU1 intron is located in the nuclear SSU rDNA and has an unusual twin-ribozyme organization, One of the ribozymes (DiGIR2) catalyses intron excision and exon ligation. The other ribozyme (DiGIR1), which along with the endonuclease-encoding I-DirI open reading frame (ORF) is inserted in DiGIR2, carries out hydrolysis at internal processing sites (IPS1 and IPS2) located at its 3' end. Examination of the in vivo expression ofD1SSU1 shows that after excision, DiSSU1 is matured further into the I-DirI mRNA by internal DiGIR1-catalysed cleavage upstream of the ORF 5' end, aswell as truncation and polyadenylation downstream of the ORE 3' end. A spliceosomal intron, the first to be reported within a group I intron and the rDNA, is removed before the I-DirI mRNA associates with the polysomes, Taken together, our results imply that DiSSU1 uses a unique combination of intron-supplied ribozyme activity and adaptation to the general RNA polymerase II pathway of mRNA expression to allow a protein to be produced from the RNA polymerase I-transcribed rDNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:16:11