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Titolo:
Regulation of connexin32 and connexin43 gene expression by DNA methylationin rat liver cells
Autore:
Piechocki, MP; Burk, RD; Ruch, RJ;
Indirizzi:
Med Coll Ohio, Dept Pathol, Toledo, OH 43699 USA Med Coll Ohio Toledo OH USA 43699 Ohio, Dept Pathol, Toledo, OH 43699 USA YeshivaNYniv Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, Bronx,Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 Res Ctr, Bronx,
Titolo Testata:
CARCINOGENESIS
fascicolo: 3, volume: 20, anno: 1999,
pagine: 401 - 406
SICI:
0143-3334(199903)20:3<401:ROCACG>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAP JUNCTION PROTEIN; CARCINOGENESIS; PROMOTERS; ESTROGEN; NUMBER; CDNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Ruch, RJ Med Coll Ohio, Dept Pathol, 3055 Arlington Ave, Toledo, OH 43699 USA Med Coll Ohio 3055 Arlington Ave Toledo OH USA 43699 OH 43699 USA
Citazione:
M.P. Piechocki et al., "Regulation of connexin32 and connexin43 gene expression by DNA methylationin rat liver cells", CARCINOGENE, 20(3), 1999, pp. 401-406

Abstract

Gap junction proteins (connexins) are expressed in a cell-specific manner and expression is often reduced in neoplastic cells. We investigated the mechanisms of connexin32 (Cx32) and connexin43 (Cx43) expression in hepatic cells using MH1C1 rat hepatoma cells and freshly isolated, adult rat hepatocytes that express Cx32 but not Cx43 and WB-F344 rat liver epithelial cells that express Cx43 but not Cx32, Southern blotting after DNA restriction with MspI and HpaII indicated that two MspI/HpaII restriction sites in the Cx32 promoter (positions -147 and -847) were methylated in WB-F344 cells, but not in MH1C1 cells or hepatocytes. In contrast, an MspI/HpaII restriction site in the Cx43 promoter (position 38) was methylated in MH1C1 cells, but not in WB-F344 cells or hepatocytes, Transient transfection of the cell lines with connexin promoter-luciferase constructs indicated that the Cx32 promoter was 7-fold more active in MH1C1 cells and the Cx43 promoter was 5-foldmore active in WB-F344 cells. These results suggest that transcription of Cx32 and Cx43 in hepatic cells is controlled by promoter methylation and bycell-specific transcription factors. Similar mechanisms may be involved inthe reduced expression of these genes frequently observed in neoplastic cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 06:30:13