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Titolo:
Recovery of RNA synthesis from the DHFR gene following UV-irradiation precedes the removal of photolesions from the transcribed strand
Autore:
Ljungman, M;
Indirizzi:
Univrbor,igan, Ctr Comprehens Canc, Div Canc Biol, Dept Radiat Oncol, Ann A Univ Michigan Ann Arbor MI USA 48109 Canc Biol, Dept Radiat Oncol, Ann A
Titolo Testata:
CARCINOGENESIS
fascicolo: 3, volume: 20, anno: 1999,
pagine: 395 - 399
SICI:
0143-3334(199903)20:3<395:RORSFT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIHYDROFOLATE-REDUCTASE GENE; HAMSTER OVARY CELLS; POLYMERASE-II TRANSCRIPTION; CYCLOBUTANE PYRIMIDINE DIMER; PREFERENTIAL DNA-REPAIR; COCKAYNE-SYNDROME CELLS; 6-4 PHOTOPRODUCTS; COUPLED REPAIR; XERODERMA PIGMENTOSUM; DIFFERENTIAL REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Ljungman, M UnivCCGC,1500, Ctr Comprehens Canc, Div Canc Biol, Dept RadiatOncol, 4306 Univ Michigan 4306 CCGC,1500 E Med Ctr Dr Ann Arbor MI USA 48109
Citazione:
M. Ljungman, "Recovery of RNA synthesis from the DHFR gene following UV-irradiation precedes the removal of photolesions from the transcribed strand", CARCINOGENE, 20(3), 1999, pp. 395-399

Abstract

It is thought that recovery of RNA synthesis following UV-irradiation is closely related to the removal of UV-induced lesions from the transcribed strand of active genes. To test this hypothesis, nascent RNA synthesis from three different locations within the DHFR gene in CHO cells was assessed following exposure to UV light (254 nm), Using both in vivo RNA labeling as well as the nuclear run-on technique, it was found that RNA synthesis from the middle and the 3'-end of the gene was inhibited within 20 min by similar to 30 and 70%, respectively, while RNA synthesis from the 5'-end of the DHFR gene was enhanced, RNA synthesis from the middle portion of the gene fully recovered within 30-45 min of post-UV incubation, while recovery was slower from the 3'-end of the gene. Compared with previously published data forthe kinetics of removal of UV-induced DNA lesions from the 5'-half of the DHFR gene in these cells, it is concluded that RNA synthesis resumed significantly faster in this region than could be accounted for by the removal ofphotolesions from the transcribed strand. Thus, although RNA synthesis wasinitially inhibited by UV-induced photolesions, the results suggest that RNA polymerase II was able to bypass these lesions prior to their removal.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:23:18