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Titolo:
Fluorescence in situ hybridization analysis shows the frequent occurrence of 14q32.3 rearrangements with involvement of immunoglobulin switch regionsin myeloma cell lines
Autore:
Kuipers, J; Vaandrager, JW; Weghuis, DO; Pearson, PL; Scheres, J; Lokhorst, HM; Clevers, H; Bast, BJEG;
Indirizzi:
Univ Utrecht, Dept Immunol, Utrecht, Netherlands Univ Utrecht Utrecht Netherlands ht, Dept Immunol, Utrecht, Netherlands Univ Utrecht, Dept Haematol, Utrecht, Netherlands Univ Utrecht Utrecht Netherlands t, Dept Haematol, Utrecht, Netherlands Univ Utrecht, Dept Human Genet, Utrecht, Netherlands Univ Utrecht Utrecht Netherlands Dept Human Genet, Utrecht, Netherlands Leiden Univ, Dept Pathol, Leiden, Netherlands Leiden Univ Leiden Netherlands n Univ, Dept Pathol, Leiden, Netherlands
Titolo Testata:
CANCER GENETICS AND CYTOGENETICS
fascicolo: 2, volume: 109, anno: 1999,
pagine: 99 - 107
SICI:
0165-4608(199903)109:2<99:FISHAS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MULTIPLE-MYELOMA; CHROMOSOMAL TRANSLOCATIONS; KARYOTYPE ABNORMALITIES; EPSILON-GENES; LEUKEMIA; LOCUS; LYMPHOMA; MALIGNANCIES; BREAKPOINTS; DUPLICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Kuipers, J Univ Utrecht Hosp, Dept Immunol, POB 85500, NL-3508 GA Utrecht,Netherlands Univ Utrecht Hosp POB 85500 Utrecht Netherlands NL-3508 GA nds
Citazione:
J. Kuipers et al., "Fluorescence in situ hybridization analysis shows the frequent occurrence of 14q32.3 rearrangements with involvement of immunoglobulin switch regionsin myeloma cell lines", CANC GENET, 109(2), 1999, pp. 99-107

Abstract

In many B-cell malignancies, 14q32.3 chromosomal rearrangements involving the immunoglobulin heavy chain (IgH) locus have been shown to be pathognomonic for the disease. Although in myeloma heterogeneous and complex karyotypes are found, 14q32.3 translocations are prominent. However, owing to the telomeric position of the IgH locus, 14q32.3 translocations may be easily missed, We established fluorescence in situ hybridization (FISH) assays on chromosomes and DNA fibers to determine both the occurrence of 14q32.3 rearrangements in myeloma cell lines and the precise localization of the breakpoints in the IgH locus, Our results show that 14q32.3 chromosomal rearrangements are present in almost every myeloma cell line analyzed (17 of 19, 89%). Breakpoint analysis of the lines harboring one or more 14q32.3 rearrangements with the use of fiber-FISH revealed the involvement of switch regions in the IgH locus in 11 of 17 cell lines. Remarkably, pseudogamma genes without switch regions were involved in 3 of 17 cell lines, all derived from IgAmyelomas. Three of 17 cell lines contained breakpoints outside a sn itch or immunoglobulin heavy chain constant region. The almost ubiquitous presence of 14q32.3 rearrangements suggests an obligatory role in the development of myeloma. The high incidence of breakpoints involving switch regions indicates an oncogenic event in a late stage of B-cell differentiation. (C) Elsevier Science Inc., 1999. All rights reserved.

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Documento generato il 24/11/20 alle ore 07:27:05