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Titolo:
Cyclooxygenase 2 is up-regulated and localized to macrophages in the intestine of Min mice
Autore:
Hull, MA; Booth, JK; Tisbury, A; Scott, N; Bonifer, C; Markham, AF; Coletta, PL;
Indirizzi:
StEnglandUniv Hosp, Dept Histopathol, Div Med, Leeds LS9 7TF, W Yorkshire,St James Univ Hosp Leeds W Yorkshire England LS9 7TF S9 7TF, W Yorkshire, Univ Leeds, Mol Med Unit, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England
Titolo Testata:
BRITISH JOURNAL OF CANCER
fascicolo: 9-10, volume: 79, anno: 1999,
pagine: 1399 - 1405
SICI:
0007-0920(199903)79:9-10<1399:C2IUAL>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN COLON-CANCER; NONSTEROIDAL ANTIINFLAMMATORY DRUGS; FAMILIAL ADENOMATOUS POLYPOSIS; PROSTAGLANDIN G/H SYNTHASE-1; COLORECTAL-CANCER; APC GENE; MAJOR MODIFIER; H SYNTHASE-2; MOUSE; EXPRESSION;
Keywords:
adenoma; cyclooxygenase; immunohistochemistry; macrophage; Min mouse; prostaglandin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Hull, MA St,James Univ Hosp, Dept Histopathol, Div Med, Clin Sci Bldg, Leeds LS9 7TF St James Univ Hosp Clin Sci Bldg Leeds W Yorkshire England LS9 7TF
Citazione:
M.A. Hull et al., "Cyclooxygenase 2 is up-regulated and localized to macrophages in the intestine of Min mice", BR J CANC, 79(9-10), 1999, pp. 1399-1405

Abstract

Expression of cyclooxygenase 2 (COX-2) is believed to play an important role in adenoma formation in murine polyposis models, and inhibition of COX-2activity may, at least, partly explain the chemopreventative activity of non-steroidal anti-inflammatory drugs against colorectal cancer in humans. However, the mechanism by which COX-2 acts in intestinal tumorigenesis remains unresolved because of conflicting data on the cellular localization of COX-2 in intestinal mucosa. Using immunohistochemistry with specific COX-2 antiserum, we have shown that COX-2 protein is localized to interstitial cells at the base of and within adenomas of the small and large intestine of multiple intestinal neoplasia (Min) mice. No COX-2 staining was observed in dysplastic epithelial cells within adenomas or in histologically normal epithelium. Moreover, COX-2 staining was observed in lamina propria cells of histologically normal intestine of Min mice. No staining was demonstrated inwild-type littermates, The rat monoclonal antibody F4/80 was used to show that COX-2-positive cells represented a subset of the macrophage populationpresent in the intestine of Min mice. Localization of COX-2 to macrophagesimplies a paracrine effect of COX-2 function on epithelial cells in adenomas and also on histologically normal epithelium. Up-regulation of COX-2 expression in lamina propria macrophages may precede loss of the second functional Ape allele in epithelial cells before adenoma formation in the Min mouse model of intestinal tumorigenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 07:12:27