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Titolo:
Mechanisms of apoptosis in PC12 cells irreversibly differentiated with nerve growth factor and cyclic AMP
Autore:
Lambeng, N; Michel, PP; Brugg, B; Agid, Y; Ruberg, M;
Indirizzi:
Hop La Pitie Salpetriere, INSERM, U289, F-75013 Paris, France Hop La PitieSalpetriere Paris France F-75013 289, F-75013 Paris, France
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1, volume: 821, anno: 1999,
pagine: 60 - 68
SICI:
0006-8993(19990306)821:1<60:MOAIPC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; CERAMIDE INDUCES APOPTOSIS; ALZHEIMERS-DISEASE; TRANSCRIPTION FACTOR; SYMPATHETIC NEURONS; PHEOCHROMOCYTOMA CELLS; NUCLEAR TRANSLOCATION; FACTOR DEPRIVATION; HYDROGEN-PEROXIDE; TROPHIC SUPPORT;
Keywords:
PC12 cell; trophic factor withdrawal; ceramide; free radical; nuclear factor-kappa B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Ruberg, M Hop La Pitie Salpetriere, INSERM, U289, 47 Blvd Hop, F-75013 Paris, France Hop La Pitie Salpetriere 47 Blvd Hop Paris France F-75013 rance
Citazione:
N. Lambeng et al., "Mechanisms of apoptosis in PC12 cells irreversibly differentiated with nerve growth factor and cyclic AMP", BRAIN RES, 821(1), 1999, pp. 60-68

Abstract

PC12 cells treated with cAMP become irreversibly differentiated and die byapoptosis when deprived of trophic support, instead of dedifferentiating and reentering the cell cycle. To approach the molecular mechanism underlying the cAMP-induced switch from differentiation/proliferation to apoptosis, we compared three sequential markers of a candidate apoptogenic signal transduction pathway (ceramide, free radicals and NF-kappa B), after trophic factor withdrawal in PC12 cells before and after irreversible differentiation. Serum withdrawal increased ceramide and free radical production regardless of the state of differentiation of the cells. It was followed by cell death, however, only in the absence of NGF and/or cAMP, and was no longer required for apoptosis in NGF/cAMP-differentiated cells. NGF and cAMP withdrawal sufficed. NF-kappa B was activated by NGF withdrawal in reversibly differentiated PC12 cells during dedifferentiation and reentry into the cell cycle, whereas in NGF/cAMP-differentiated cells, it was activated, at a late stage of the apoptotic process, concomitantly with cell death. These results show that a serum factor inhibits ceramide-dependent apoptosis upstream of ceramide and free radical production, whereas NGF- and cAMP-dependent mechanisms inhibit apoptosis either downstream or parallel to these events. After terminal differentiation by cAMP, apoptosis appears to be initiated from the second site, consistent with the serum independence of these cells and the absence of ceramide and free radical production when the NGF/cAMP-dependent inhibitions are released. The differential regulation of NF-kappa B appears to be an important step in the switch from mitosis to apoptosis that occurs during irreversible differentiation of PC12 cells by cAMP. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:40:18