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Titolo:
Role of cholesterol ester mass in regulation of secretion of ApoB100 lipoprotein particles by hamster hepatocytes and effects of statins on that relationship
Autore:
Zhang, ZJ; Cianflone, K; Sniderman, AD;
Indirizzi:
McGillnadav, Ctr Hlth, Mike Rosenbloom Lab Cardiovasc Res, Montreal, PQ, Ca McGill Univ Montreal PQ Canada loom Lab Cardiovasc Res, Montreal, PQ, Ca
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 3, volume: 19, anno: 1999,
pagine: 743 - 752
SICI:
1079-5642(199903)19:3<743:ROCEMI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA; INCREASED HEPATIC SECRETION; APOLIPOPROTEIN-B SECRETION; HEPG2 CELLS; ENDOPLASMIC-RETICULUM; RECEPTOR ACTIVITY; BIOSYNTHESIS; B-100; LIVER;
Keywords:
hepatocytes; lipoprotein; cholesterol ester;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Sniderman, AD Royalntreal,ia Hosp, Mike Rosenbloom Lab Cardiovasc Res, 687Pine Ave W, Mo Royal Victoria Hosp 687 Pine Ave W Montreal PQ Canada H3A 1A1
Citazione:
Z.J. Zhang et al., "Role of cholesterol ester mass in regulation of secretion of ApoB100 lipoprotein particles by hamster hepatocytes and effects of statins on that relationship", ART THROM V, 19(3), 1999, pp. 743-752

Abstract

Our understanding of the factors that regulate the secretion of apoB100 Lipoproteins remains incomplete with considerable debate as to the role, if any, for cholesterol ester in this process. This study examines this issue in primary cultures of hamster hepatocytes, a species in which both cholesterol and apoB100 metabolism are very similar to man. Addition of oleate to medium increased the mass of triglyceride and cholesterol ester within the hepatocyte and also increased the secretion of triglycerides, cholesterol ester, and apoB 100 into the medium. Next, the responses of hamster hepatocytes to addition of either an HMG-CoA reductase inhibitor (lovastatin) or an acyl-CoA cholesterol acyltransferase inhibitor (58-035) to the medium, withor without added oleate, were determined. Effects of either agent were only evident in the oleate-supplemented medium in which cholesterol ester masshad been increased above basal. If oleate was not added to the medium, neither agent reduced apoB100 secretion; equally important, over the 24-hour incubation, neither agent, at the concentration used, produced any detectable change in intracellular cholesterol ester mass. However, in contrast to the estimates of mass, which were unchanged, under the same conditions radioisotopic estimates of cholesterol ester synthesis were markedly reduced. Any conclusion as to the relation of cholesterol eater mass to apoB100 secretion would therefore depend on which of the 2 methods was used. Overall, thedata indicate a close correlation between the mass of cholesterol ester within the hepatocyte and apoB100 secretion from it and they go far to explain previous apparently contradictory data as to this relation. More importantly, though, taken with other available data, they indicate that the primary response of the liver to increased delivery of lipid is increased secretion rather than decreased uptake. These results point, therefore, to a hierarchy of hepatic responses to increased flux of fatty acids and increased synthesis of cholesterol that in turn suggests a more dynamic model of cholesterol homeostasis in the liver than has been appreciated in the past.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:44:32