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Titolo:
Lipid transfer inhibitor protein defines the participation of lipoproteinsin lipid transfer reactions - CETP has no preference for cholesteryl esters in HDL versus LDL
Autore:
Serdyuk, AP; Morton, RE;
Indirizzi:
ClevelandAClin Fdn, Lerner Res Inst, Dept Cell Biol, Cleveland, OH 44195 US Cleveland Clin Fdn Cleveland OH USA 44195 ll Biol, Cleveland, OH 44195 US Natl Res Ctr Prevent Med, Dept Biochem, Moscow, Russia Natl Res Ctr Prevent Med Moscow Russia ed, Dept Biochem, Moscow, Russia
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 3, volume: 19, anno: 1999,
pagine: 718 - 726
SICI:
1079-5642(199903)19:3<718:LTIPDT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DENSITY-LIPOPROTEINS; APOLIPOPROTEIN-A-I; TRIACYLGLYCEROL-RICH LIPOPROTEINS; HUMAN-PLASMA; DEFICIENT PLASMA; MASS-TRANSFER; NORMOLIPIDEMIC SUBJECTS; EXCHANGE PROTEIN; HUMAN-SERUM; TRIGLYCERIDE;
Keywords:
cholesteryl ester transfer protein; lipid transfer inhibitor protein; lipoprotein preference; cholesteryl ester; heteroexchange;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Morton, RE Cleveland Clin Fdn, Lerner Res Inst, Dept Cell Biol, NC 10,9500Euclid Ave, Cleveland Clin Fdn NC 10,9500 Euclid Ave Cleveland OH USA 44195
Citazione:
A.P. Serdyuk e R.E. Morton, "Lipid transfer inhibitor protein defines the participation of lipoproteinsin lipid transfer reactions - CETP has no preference for cholesteryl esters in HDL versus LDL", ART THROM V, 19(3), 1999, pp. 718-726

Abstract

Cholesteryl eater transfer protein (CETP) catalyzes the net transfer of cholesteryl ester (CE) between lipoproteins in exchange for triglyceride (heteroexchange). It is generally held that CETP primarily associates with HDL and preferentially transfers lipids from this lipoprotein fraction. This isillustrated in normal plasma where HDL is the primary donor of the CE transferred to VLDL by CETP. However, in plasma deficient in lipid transfer inhibitor protein (LTIP) activity, HDL and LDL are equivalent donors of CE to VLDL (Arterioscler Thromb Vasc Biol. 1997;17:1716-1724). Thus, we have hypothesized that the preferential transfer of CE from HDL. in normal plasma isa consequence of LTIP activity and not caused by a preferential CETP-HDL interaction. We have tested this hypothesis in lipid mass transfer assays with partially purified CETP and LTIP, and isolated lipoproteins. With a physiological mixture of lipoproteins, the preference ratio (PR, ratio of CE mass transferred from a lipoprotein to VLDL versus its CE content) for HDL and LDL in the presence of CETP alone was approximate to 1 (ie, no preference). Fourfold variations in the LDL/HDL ratio or in the levels of HDL in the assay did not result in significant preferential transfer from any lipoprotein. On addition of LTIP, the PR for HDL was increased up to 2-fold and that for LDL decreased in a concentration-dependent manner. Under all conditions where LDL and HDL levels were varied, LTIP consistently resulted in a PR>1 for CE transfer from HDL. Short-term experiments with radiolabeled lipoproteins and either partially purified or homogenous CETP confirmed these observations and further demonstrated that CETP has a strong predilection tomediate homoexchange (bidirectional transfer of the same lipid) rather than heteroexchange (CE for TG); LTLP had no effect on the selection of CE or TG by CETP or its mechanism of action. We conclude, in contrast to current opinion, that CETP has no preference for CE in HDL versus LDL, suggesting that the previously reported stable binding of CETP to HDL does not result in selective transfer from this lipoprotein. These data suggest that LTIP isresponsible for the preferential transfer of CE from HDL that occurs in plasma. CETP and LTIP cooperatively determine the extent of CETP-mediated remodeling of individual lipoprotein fractions.

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Documento generato il 10/07/20 alle ore 07:40:13