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Titolo:
Contribution of mitochondrial proton leak to respiration rate in working skeletal muscle and liver and to SMR
Autore:
Rolfe, DFS; Newman, JMB; Buckingham, JA; Clark, MG; Brand, MD;
Indirizzi:
Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England Univ Cambridge Cambridge England CB2 1QW hem, Cambridge CB2 1QW, England Univ Tasmania, Div Biochem, Hobart, Tas 7001, Australia Univ Tasmania Hobart Tas Australia 7001 chem, Hobart, Tas 7001, Australia
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 3, volume: 45, anno: 1999,
pagine: C692 - C699
SICI:
0363-6143(199903)45:3<C692:COMPLT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATP TURNOVER; CONTRACTION; METABOLISM; HEPATOCYTES; PERFORMANCE;
Keywords:
standard metabolic rate; proton pumping; redox energy; contracting skeletal muscle; working liver cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Rolfe, DFS Univstraliagong, Dept Biomed Sci, Northfields Ave, Wollongong, NSW 2522, Au Univ Wollongong Northfields Ave Wollongong NSW Australia 2522 u
Citazione:
D.F.S. Rolfe et al., "Contribution of mitochondrial proton leak to respiration rate in working skeletal muscle and liver and to SMR", AM J P-CELL, 45(3), 1999, pp. C692-C699

Abstract

Proton pumping across the mitochondrial inner membrane and proton leak back through the natural proton conductance pathway make up a futile cycle that dissipates redox energy. We measured respiration and average mitochondrial membrane potential in perfused rat hindquarter with maximal tetanic contraction of the left gastrocnemius-soleus-plantaris muscle group, and we estimate that the mitochondrial proton cycle accounted for 34% of the respiration rate of the preparation. Similar measurements in rat hepatocytes given substrates to cause a high rate of gluconeogenesis and ureagenesis showed that the proton cycle accounted for 22% of the respiration rate of these cells. Combining these in vitro values with literature values for the contribution of skeletal muscle and liver to standard metabolic rate (SMR), we calculate that the proton cycle in working muscle and Liver may account for 15% of SMR in vivo. Although this value is less than the 20% of SMR we calculated previously using data from resting skeletal muscle and hepatocytes, it is still large, and we conclude that the futile proton cycle is a major contributor to SMR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 10:18:48