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Titolo:
Overexpression of recombinant proteins with a C-terminal thiocarboxylate: Implications for protein semisynthesis and thiamin biosynthesis
Autore:
Kinsland, C; Taylor, SV; Kelleher, NL; McLafferty, FW; Begley, TP;
Indirizzi:
Cornell Univ, Baker Lab, Dept Chem & Chem Biol, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 t Chem & Chem Biol, Ithaca, NY 14853 USA
Titolo Testata:
PROTEIN SCIENCE
fascicolo: 8, volume: 7, anno: 1998,
pagine: 1839 - 1842
SICI:
0961-8368(199808)7:8<1839:OORPWA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
UNPROTECTED PEPTIDE SEGMENTS; RESOLUTION;
Keywords:
C-terminal thiocarboxylate; protein semisynthesis; recombinant proteins; thiamin biosynthesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Begley, TP Cornell Univ, Baker Lab, Dept Chem & Chem Biol, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 em Biol, Ithaca, NY 14853 USA
Citazione:
C. Kinsland et al., "Overexpression of recombinant proteins with a C-terminal thiocarboxylate: Implications for protein semisynthesis and thiamin biosynthesis", PROTEIN SCI, 7(8), 1998, pp. 1839-1842

Abstract

A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multi-milligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for proteinsemisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:06:33