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Titolo:
Roles of Na+-Ca2+ exchange and of mitochondria in the regulation of presynaptic Ca2+ and spontaneous glutamate release
Autore:
Scotti, AL; Chatton, JY; Reuter, H;
Indirizzi:
Univ Bern, Dept Pharmacol, CH-3010 Bern, Switzerland Univ Bern Bern Switzerland CH-3010 Pharmacol, CH-3010 Bern, Switzerland
Titolo Testata:
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
fascicolo: 1381, volume: 354, anno: 1999,
pagine: 357 - 364
SICI:
0962-8436(19990228)354:1381<357:RONEAO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; ROOT GANGLION NEURONS; SYNAPTIC TRANSMISSION; INTRACELLULAR CALCIUM; TRANSMITTER RELEASE; HIPPOCAMPAL-NEURONS; NERVE-ENDINGS; RAT-BRAIN; CELLS; LOADS;
Keywords:
Na+-Ca2+ exchange; mitochondria; hippocampal cells; spontaneous excitatory postsynaptic currents (sEPSC); glutamate release; presynaptic Ca2+ concentration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Reuter, H Univ Bern, Dept Pharmacol, Friedbuhlstr 49, CH-3010 Bern, Switzerland Univ Bern Friedbuhlstr 49 Bern Switzerland CH-3010 Switzerland
Citazione:
A.L. Scotti et al., "Roles of Na+-Ca2+ exchange and of mitochondria in the regulation of presynaptic Ca2+ and spontaneous glutamate release", PHI T ROY B, 354(1381), 1999, pp. 357-364

Abstract

The release of neurotransmitter from presynaptic terminals depends on an increase in the intracellular Ca2+ concentration ([Ca2+](i)). In addition tothe opening of presynaptic Ca2+ channels during excitation, other Ca2+ transport systems may be involved in changes in [Ca2+]i. We have studied the regulation of [Ca2+](i) in nerve terminals of hippocampal cells in culture by the Na+-Ca2+ exchanger and by mitochondria. In addition, we have measuredchanges in the frequency of spontaneous excitatory postsynaptic currents (sEPSC) before and after the inhibition of the exchanger and of mitochondrial metabolism. We found rather heterogeneous [Ca2+](i) responses of individual presynaptic terminals after inhibition of Na+-Ca2+ exchange. The increase in [Ca2+](i) became more uniform and much larger after additional treatment of the cells with mitochondrial inhibitors. Correspondingly, sEPSC frequencies changed very little when only Na+-Ca2+ exchange was inhibited, hut increased dramatically after additional inhibition of mitochondria. Our results provide evidence for prominent roles of Na+-Ca2+ exchange and mitochondria in presynaptic Ca2+ regulation and spontaneous glutamate release.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:44:06