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Titolo:
Dietary fish oils inhibit early events in the assembly of very low densitylipoproteins and target apoB for degradation within the rough endoplasmic reticulum of hamster hepatocytes
Autore:
Kendrick, JS; Higgins, JA;
Indirizzi:
Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, Univ Sheffield Sheffield S Yorkshire England S10 2TN 10 2TN, S Yorkshire,
Titolo Testata:
JOURNAL OF LIPID RESEARCH
fascicolo: 3, volume: 40, anno: 1999,
pagine: 504 - 514
SICI:
0022-2275(199903)40:3<504:DFOIEE>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-3 FATTY-ACIDS; APOLIPOPROTEIN-B SECRETION; HEP G2 CELLS; TRIGLYCERIDE TRANSFER PROTEIN; UBIQUITIN-PROTEASOME PATHWAY; CULTURED RAT HEPATOCYTES; INTRACELLULAR DEGRADATION; RABBIT HEPATOCYTES; ER-60 PROTEASE; HEPATOMA-CELLS;
Keywords:
liver hepatocytes; hamster; diet; fish oil; sunflower oil; apolipoprotein B; degradation; secretion; o-phenanthroline; ALLN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Higgins, JA Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN,S Yorkshire, Univ Sheffield Sheffield S Yorkshire England S10 2TN rkshire,
Citazione:
J.S. Kendrick e J.A. Higgins, "Dietary fish oils inhibit early events in the assembly of very low densitylipoproteins and target apoB for degradation within the rough endoplasmic reticulum of hamster hepatocytes", J LIPID RES, 40(3), 1999, pp. 504-514

Abstract

Dietary fish oils inhibited secretion and stimulated intracellular degradation of apolipoprotein (apo)B in hamster hepatocytes, while dietary sunflower oils stimulated secretion and had no effect on degradation of apoB, To investigate the inh acellular site at which fish oils act, we have made use of our previous observations that inhibition of degradation by N-acetyl-leucyl-leucyl-norleucinal (ALLN) results in accumulation of apoB in the trans-Golgi membrane and does not stimulate secretion, while inhibition of degradation by o-phenanthroline results in accumulation of apoB in the rough endoplasmic reticulum membrane and stimulates secretion, Thm, ALLN protects apoB which has been diverted from secretion and o-phenanthroline protects apoBwhich is targetted for secretion. Addition of o-phenantholine to the incubation medium of hepatocytes from fish oil-fed hamsters inhibited degradation of apoB and stimulated its secretion in particles of the density of VLDL,while addition of ALLN had no effect.jlr These observations suggest that dietary fish oils reversibly inhibit early steps in the assembly of very lowdensity Lipoprotein precursors and target apoB for degradation in the rough endoplasmic reticulum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 09:53:35