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Titolo:
Development of porcine adenovirus-3 as an expression vector
Autore:
Reddy, PS; Idamakanti, N; Hyun, BH; Tikoo, SK; Babiuk, LA;
Indirizzi:
Univ Saskatchewan, Vet Infect Dis Org, Saskatoon, SK S7N 0W0, Canada Univ Saskatchewan Saskatoon SK Canada S7N 0W0 skatoon, SK S7N 0W0, Canada Natl Inst Vet Res, Kuyunggi Do, South Korea Natl Inst Vet Res Kuyunggi Do South Korea Res, Kuyunggi Do, South Korea
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 80, anno: 1999,
parte:, 3
pagine: 563 - 570
SICI:
0022-1317(199903)80:<563:DOPAAA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT ADENOVIRUS; SEQUENCE-ANALYSIS; TYPE-3; CONSTRUCTION; EFFICIENT; VIRUS; NUCLEOTIDE; PROTEIN; REGIONS; GENOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Reddy, PS Univ Saskatchewan, Vet Infect Dis Org, Saskatoon, SK S7N 0W0, Canada Univ Saskatchewan Saskatoon SK Canada S7N 0W0 K S7N 0W0, Canada
Citazione:
P.S. Reddy et al., "Development of porcine adenovirus-3 as an expression vector", J GEN VIROL, 80, 1999, pp. 563-570

Abstract

Porcine adenovirus-3 (PAV-3) was developed as an expression vector using homologous recombination in Escherichia coli BJ 5183, As a prerequisite, thecomplete genome of PAV-3 was first introduced as a Pad restriction fragment into a bacterial plasmid. The plasmid, when Pad restricted and transfected into swine testicular cells, produces an infectious virus. The potential of this procedure was demonstrated by the construction of several PAV-3 recombinants. Part of the E3 region, which is nonessential for virus replication under cell culture conditions, was identified and deleted from the virusgenome. The gene for glycoprotein D (gD) of pseudorabies virus (PRV), which elicits PRV-neutralizing antibodies in pigs, was cloned and expressed from the E3 region of PAV-3, A 50 kDa polypeptide was identified in recombinant PAV-3-infected cell lysates by immunoprecipitation assays using go-specific monoclonal antibodies. In another experiment, a region between the rightinverted terminal repeat and the promoter of the E4 region was used to clone and express the chloramphenicol acetyltransferase (CAT) gene under the control of SV40 immediate early promoter. CAT gene expression was observed irrespective of the orientation of the CAT gene. These results indicate thatthe helper-independent recombinant PAV-3 could be used as an expression vector and has potential as a recombinant vaccine vector in pigs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 08:19:00