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Titolo:
CCAAT/enhancer-binding proteins - A role in regulation of human involucrinpromoter response to phorbol ester
Autore:
Agarwal, C; Efimova, T; Welter, JF; Crish, JF; Eckert, RL;
Indirizzi:
Case4106tern Reserve Univ, Sch Med, Dept Physiol & Biophys, Cleveland, OH 4 Case Western Reserve Univ Cleveland OH USA 44106 Biophys, Cleveland, OH 4 Case Western Reserve Univ, Sch Med, Dept Dermatol, Cleveland, OH 44106 USACase Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA CaseSAestern Reserve Univ, Sch Med, Dept Reprod Biol, Cleveland, OH 44106 U Case Western Reserve Univ Cleveland OH USA 44106 l, Cleveland, OH 44106 U Case Western Reserve Univ, Sch Med, Dept Biochem, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Sch Med, Dept Oncol, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 10, volume: 274, anno: 1999,
pagine: 6190 - 6194
SICI:
0021-9258(19990305)274:10<6190:CP-ARI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
KERATIN INTERMEDIATE FILAMENTS; TRANSCRIPTION FACTORS C/EBP; ADIPOCYTE DIFFERENTIATION; TRANSGENIC MICE; EXPRESSION; GENE; ENVELOPE; FAMILY; ALPHA; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Eckert, RL Caselbertern Reserve Univ, Sch Med, Dept Physiol & Biophys, Rm 532,2109 Ade Case Western Reserve Univ Rm 532,2109 Adelbert Rd Cleveland OHUSA 44106
Citazione:
C. Agarwal et al., "CCAAT/enhancer-binding proteins - A role in regulation of human involucrinpromoter response to phorbol ester", J BIOL CHEM, 274(10), 1999, pp. 6190-6194

Abstract

The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) is a potent inducer of keratinocyte differentiation and of involucrin gene expression. In the present study we show that a CCAAT/enhancer-binding protein (C/EBP) site in the proximal regulatory region is required for the phorbol ester response. Mutation of the C/EBP site results in the loss of basal and TPA-responsive activity. Gel mobility supershift analysis shows that C/EBP alpha binding to this site is increased by TPA treatment. Moreover, cotransfection of the human involucrin reporter plasmid with C/EBP alpha increases promoter activity to an extent comparable with TPA treatment. Mutation of the C/EBP-binding site eliminates these responses. Transfection experiments using GADD153 to create C/EBP-null conditions confirm that C/EBP factors are absolutely required for promoter activity and TPA responsiveness. C/EBP alpha and C/EBP delta inhibit both TPA- and C/EBP alpha-dependent promoter activation, indicating functional differences among C/EBP family members. These results suggest that C/EBP transcription factor activity is necessary for basal promoter activity and TPA response of the involucrin gene.

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Documento generato il 25/09/20 alle ore 00:42:07