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Titolo:
Ceramide induces cytochrome c release from isolated mitochondria - Importance of mitochondrial redox state
Autore:
Ghafourifar, P; Klein, SD; Schucht, O; Schenk, U; Pruschy, M; Rocha, S; Richter, C;
Indirizzi:
Swiss Fed Inst Technol, Biochem Lab 1, CH-8092 Zurich, Switzerland Swiss Fed Inst Technol Zurich Switzerland CH-8092 92 Zurich, Switzerland Univ Zurich Hosp, Dept Radiat Oncol, CH-8092 Zurich, Switzerland Univ Zurich Hosp Zurich Switzerland CH-8092 CH-8092 Zurich, Switzerland
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 10, volume: 274, anno: 1999,
pagine: 6080 - 6084
SICI:
0021-9258(19990305)274:10<6080:CICCRF>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-LIVER MITOCHONDRIA; CELL-PERMEABLE CERAMIDE; INDUCED APOPTOSIS; TNF-ALPHA; KAPPA-B; MECHANISM; DEATH; REQUIREMENT; GENERATION; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Richter, C Swissland Inst Technol, Biochem Lab 1, Univ Str 16, CH-8092 Zurich, Switzer Swiss Fed Inst Technol Univ Str 16 Zurich Switzerland CH-8092
Citazione:
P. Ghafourifar et al., "Ceramide induces cytochrome c release from isolated mitochondria - Importance of mitochondrial redox state", J BIOL CHEM, 274(10), 1999, pp. 6080-6084

Abstract

In the present study we show that N-acetylsphingosine (C-2-ceramide), N-hexanoylsphingosine (C-6-ceramide), and, to a much lesser extent, C-2-dihydroceramide induce cytochrome c (cyto c) release from isolated rat liver mitochondria. Ceramide-induced cyto c release is prevented by preincubation of mitochondria with a low concentration (40 nM) of Bcl-2. The release takes place when cyto c is oxidized but not when it is reduced. Upon cyto c loss, mitochondrial oxygen consumption, mitochondrial transmembrane potential (Delta Psi), and Ca2+ retention are diminished. Incubation with Bcl-2 prevents,and addition of cyto c reverses the alteration of these mitochondrial functions. In ATP-energized mitochondria, ceramides do not alter Delta Psi, neither when cyto c is oxidized nor when it is reduced, ruling out a nonspecific disturbance by ceramides of mitochondrial membrane integrity. Furthermore, ceramides decrease the reducibility of cyto c. We conclude that the apoptogenic properties of ceramides are in part mediated via their interaction with mitochondrial cyto c followed by its release and that the redox state of cyto c influences its detachment by ceramide from the inner mitochondrial membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:56:30