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Titolo:
Purification and characterization of rat hippocampal CA3-dendritic spines associated with mossy fiber terminals
Autore:
Kiebler, MA; Lopez-Garcia, JC; Leopold, PL;
Indirizzi:
European Mol Biol Lab, Cell Biol Programme, D-69117 Heidelberg, Germany European Mol Biol Lab Heidelberg Germany D-69117 117 Heidelberg, Germany CSIC, Inst Cajal, E-28002 Madrid, Spain CSIC Madrid Spain E-28002CSIC, Inst Cajal, E-28002 Madrid, Spain Cornell Univ, Coll Med, Dept Med, New York, NY USA Cornell Univ New York NY USA Univ, Coll Med, Dept Med, New York, NY USA Cornell Univ, Coll Med, Dept Biochem, New York, NY USA Cornell Univ New York NY USA v, Coll Med, Dept Biochem, New York, NY USA
Titolo Testata:
FEBS LETTERS
fascicolo: 1, volume: 445, anno: 1999,
pagine: 80 - 86
SICI:
0014-5793(19990219)445:1<80:PACORH>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; LONG-TERM POTENTIATION; PIG CEREBRAL-CORTEX; BIOCHEMICAL-CHARACTERIZATION; SYNAPTIC PLASTICITY; DENDRITIC SPINES; PYRAMIDAL CELLS; RECEPTORS; SYNAPTOSOMES; GLUTAMATE;
Keywords:
dendritic spine; mossy fiber; hippocampus; nitric oxide synthase; electron microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Kiebler, MA Europeanrg,l Biol Lab, Cell Biol Programme, Meyerhofstr 1, D-69117 Heidelbe European Mol Biol Lab Meyerhofstr 1 Heidelberg Germany D-69117
Citazione:
M.A. Kiebler et al., "Purification and characterization of rat hippocampal CA3-dendritic spines associated with mossy fiber terminals", FEBS LETTER, 445(1), 1999, pp. 80-86

Abstract

We report a revised and improved isolation procedure for CA3-dendritic spines, most of them still in association with mossy fiber terminals resultingin a 7.5-fold enrichment over nuclei and a 29-fold enrichment over myelin. Additionally, red blood cells, medullated fibers, mitochondria and small synaptosomes were significantly depleted. We show bg high resolution electron microscopy that this subcellular fraction contains numerous dendritic spines with a rich ultrastructure, e.g. an intact spine apparatus, membranous organelles, free and membrane-bound polyribosomes, endocytic structures andmitochondria. This improved experimental system will allow us to study aspects of post-synaptic functions at the biochemical and molecular level, (C)1999 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 08:46:47