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Titolo:
Hepatocyte growth factor activator inhibitor type 2 lacking the first Kunitz-type serine proteinase inhibitor domain is a predominant product in mouse but not in human
Autore:
Itoh, H; Kataoka, H; Hamasuna, R; Kitamura, N; Koono, M;
Indirizzi:
Miyazaki Med Coll, Dept Pathol 2, Miyazaki 8891692, Japan Miyazaki Med Coll Miyazaki Japan 8891692 thol 2, Miyazaki 8891692, Japan Tokyohama, Technol, Fac Biosci & Biotechnol, Dept Life Sci, Midori Ku, Yoko Tokyo Inst Technol Yokohama Kanagawa Japan 2268501 e Sci, Midori Ku, Yoko
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 255, anno: 1999,
pagine: 740 - 748
SICI:
0006-291X(19990224)255:3<740:HGFAIT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SINGLE CHAIN FORM; PROTEASE INHIBITOR; LIGHT-CHAIN; SCATTER FACTOR; PLACENTAL BIKUNIN; MOLECULAR-CLONING; MESSENGER-RNA; EXPRESSION; PURIFICATION; PRECURSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Koono, M Miyazaki Med Coll, Dept Pathol 2, 5200 Kihara, Miyazaki 8891692, Japan Miyazaki Med Coll 5200 Kihara Miyazaki Japan 8891692 1692, Japan
Citazione:
H. Itoh et al., "Hepatocyte growth factor activator inhibitor type 2 lacking the first Kunitz-type serine proteinase inhibitor domain is a predominant product in mouse but not in human", BIOC BIOP R, 255(3), 1999, pp. 740-748

Abstract

Hepatocyte growth factor activator inhibitor type 2 (HAI-2) is a new Kunitz-type serine protease inhibitor, which is purified and cloned from human stomach cancer cell line MKN45. The mature HAI-2 protein contains two Kunitzdomains and the first domain is mainly responsible for the inhibitory activity against hepatocyte growth factor activator (HGFA). In this study, we identified the mouse homolog of HAI-2 (mHAI-2) by screening the data base ofpublic expressed sequence tag (dbEST). In addition to a full-length cDNA corresponding to human HAI-2, a shorter size of mHAI-2 cDNA was obtained from mouse kidney by reverse-transcription polymerase chain reaction (RT-PCR). Sequence analysis of this shorter cDNA revealed that the region encoding the first Kunitz domain was completely deleted. Analysis of mouse genomic DNA showed that the deleted cDNA was generated by an alternative splicing mechanism. Surprisingly, the spliced form lacking the first Kunitz domain was a predominant transcript in all tissues of mice tested but not in those of human as assessed by RT-PCR analysis. This phenomenon is also confirmed by Western blot analysis using the specific antiserum against human HAI-2 protein. These results suggest that most of HAI-2 expressed in various tissues of mice may be unable to inhibit HGFA efficiently, (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:41:34