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Titolo:
Antiapoptotic action of prolactin is associated with up-regulation of Bcl-2 and down-regulation of Bax in HC11 mouse mammary epithelial cells
Autore:
Ploszaj, T; Motyl, T; Orzechowski, A; Zimowska, W; Wareski, P; Skierski, J; Zwierzchowski, L;
Indirizzi:
Agr Univ Warsaw, Fac Med Vet, Dept Anim Physiol, PL-02787 Warsaw, Poland Agr Univ Warsaw Warsaw Poland PL-02787 Physiol, PL-02787 Warsaw, Poland Drug Inst, Flow Cytometry Lab, Warsaw, Poland Drug Inst Warsaw PolandDrug Inst, Flow Cytometry Lab, Warsaw, Poland Polish Acad Sci, Inst Genet & Anim Breeding, Jastrzebiec, Poland Polish Acad Sci Jastrzebiec Poland & Anim Breeding, Jastrzebiec, Poland
Titolo Testata:
APOPTOSIS
fascicolo: 4, volume: 3, anno: 1998,
pagine: 295 - 304
SICI:
1360-8185(199809)3:4<295:AAOPIA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CASEIN GENE-EXPRESSION; GLAND INVOLUTION; APOPTOSIS; DEATH; TISSUE; SECRETION; MILK; PROTEINASES; SURVIVAL; CULTURE;
Keywords:
apoptosis; Bax; Bcl-2; HC11; mammary gland; prolactin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Motyl, T Agr87niv Warsaw, Fac Med Vet, Dept Anim Physiol, Nowoursynowska 166, PL-027 Agr Univ Warsaw Nowoursynowska 166 Warsaw Poland PL-02787 PL-027
Citazione:
T. Ploszaj et al., "Antiapoptotic action of prolactin is associated with up-regulation of Bcl-2 and down-regulation of Bax in HC11 mouse mammary epithelial cells", APOPTOSIS, 3(4), 1998, pp. 295-304

Abstract

The effect of prolactin on apoptosis and the expression of bcl-2 and bax in HC11 mouse mammary epithelial cells were investigated. Flow cytometric analysis of Bcl-2 level (FITC-conjugated monoclonal anti-Bcl-2 antibody and FITC-conjugated monoclonal anti-IgG(1) antibody as a negative control), number of apoptotic cells and cell cycle phases (DNA stained with DAPI) was performed. Bax transcript was measured using the RT-PCR method with GAPDH serving as a reference gene. Administration of prolactin (5 mu g/ml) in the presence of insulin stimulated differentiation of mammary epithelial cells, which manifested in stopping cells at G(0)/G(1) phase, cell swelling and increase of cell number with enhanced protein content. Moreover, prolactin highly significantly reduced the extent of apoptosis of HC11 cells during 48 h of incubation. Nevertheless, the apoptotic cell number rose with increased time length of cell culture, probably due to the resulting high cell density and EGF withdrawal from the incubation medium. The antiapoptotic effect of prolactin was associated with up-regulation of bcl-2 expression, shown asan increase in cell numbers expressing this protooncogene and elevated Bcl-2 content in these cells. A negative relationship (r = -0.87, p less than or equal to 0.001) between the number of apoptotic cells and those expressing bcl-2 was also found. Prolactin administration lowered Bax transcript by68.8% and 70.7% after 3 and 6 h, respectively. In conclusion, the results presented indicate that stimulation of bcl-2 expression with simultaneous suppression of bax may be key events in the mechanism of antiapoptotic action of prolactin in HC11 mammary epithelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:35:10