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Titolo:
Use of vapor-phase acid hydrolysis for mass spectrometric peptide mapping and protein identification
Autore:
Gobom, J; Mirgorodskaya, E; Nordhoff, E; Hojrup, P; Roepstorff, P;
Indirizzi:
Odense Univ, Dept Mol Biol, DK-5230 Odense M, Denmark Odense Univ OdenseDenmark M v, Dept Mol Biol, DK-5230 Odense M, Denmark Univ,Gothenburg, Molndal Hosp, Sect Psychiat & Neurosci, Dept Clin Neurosci Univ Gothenburg Molndal Sweden S-43180 at & Neurosci, Dept Clin Neurosci
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 5, volume: 71, anno: 1999,
pagine: 919 - 927
SICI:
0003-2700(19990301)71:5<919:UOVAHF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCE DATABASES; INFORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Roepstorff, P Odense Univ, Dept Mol Biol, Campusvej 55, DK-5230 Odense M, Denmark Odense Univ Campusvej 55 Odense Denmark M Odense M, Denmark
Citazione:
J. Gobom et al., "Use of vapor-phase acid hydrolysis for mass spectrometric peptide mapping and protein identification", ANALYT CHEM, 71(5), 1999, pp. 919-927

Abstract

A method for mass spectrometric peptide mapping was developed, based on hydrolysis of a solid protein by acid vapor followed by mass spectrometric analysis of the cleavage products, The method is applicable to lyophilized samples as well as proteins present in gels after separation by SDS-PAGE. Thecleavage specificity was established using a number of standard proteins. Three different types of cleavages were observed: specific internal backbone cleavages at Asp, Ser, Thr, and Gly and N- and C-terminal sequence ladders, On the basis of the observed cleavage characteristics, a strategy for protein identification based on the peptide mass maps was developed. The identification strategy utilizes the specific internal backbone cleavages as well as the partial sequence information, obtained from the sequence ladders.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 03:56:12