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Titolo:
alpha(l)-Adrenergic stimulation of FGF-2 promoter in cardiac myocytes and in adult transgenic mouse hearts
Autore:
Detillieux, KA; Meij, JTA; Kardami, E; Cattini, PA;
Indirizzi:
Univiol,itoba, St Boniface Gen Hosp, Res Ctr, Inst Cardiovasc Sci,Dept Phys Univ Manitoba Winnipeg MB Canada R3E 3J7 r, Inst Cardiovasc Sci,Dept Phys UnivnManitoba, St Boniface Gen Hosp, Res Ctr, Inst Cardiovasc Sci,Dept Huma Univ Manitoba Winnipeg MB Canada R3E 3J7 r, Inst Cardiovasc Sci,Dept Huma
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 3, volume: 45, anno: 1999,
pagine: H826 - H833
SICI:
0363-6135(199903)45:3<H826:ASOFPI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; TRANSCRIPTION FACTOR EGR-1; SMOOTH-MUSCLE CELLS; FACTOR BFGF; FACTOR-II; RESPONSE ELEMENTS; GENE-EXPRESSION; FACTOR RECEPTOR; FACTOR RELEASE; PROLIFERATION;
Keywords:
basic fibroblast growth factor; rat fibroblast growth factor-2; gene; phenylephrine; gene transfer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Cattini, PA Univ Manitoba, Dept Physiol, 730 William Ave, Winnipeg, MB R3E3J7, Canada Univ Manitoba 730 William Ave Winnipeg MB Canada R3E 3J7 anada
Citazione:
K.A. Detillieux et al., "alpha(l)-Adrenergic stimulation of FGF-2 promoter in cardiac myocytes and in adult transgenic mouse hearts", AM J P-HEAR, 45(3), 1999, pp. H826-H833

Abstract

Fibroblast growth factor (FGF-2), a mitogenic, angiogenic, and cardioprotective agent, is reported to be released from the postnatal heart by a mechanism of transient remodeling of the sarcolemma during contraction. This release can be increased with adrenergic stimulation. RNA blotting was used toassess whether FGF-2 synthesis in neonatal rat cardiomyocytes might also be regulated by adrenergic stimulation. FGF-S RNA levels were increased after treatment with norepinephrine for 6 h or with the oc-adrenergic agonist phenylephrine for 48 h. To assess an effect on transcription, neonatal rat cardiomyocytes were transfected with a hybrid rat FGF-2 promoter/luciferase gene (-1058FGFp.luc) and treated with norepinephrine or phenylephrine for 6or 48 h, respectively. FGF-2 promoter activity was increased two- to sevenfold in an al-specific manner. Putative phenylephrine-responsive elements (PEREs) were identified at positions -780 and -761 relative to a major transcription initiation site. However, deletion analysis of -1058FGFp.luc showed that the phenylephrine response was independent of the putative PEREs, cell contraction, and Ca2+ influx. In transgenic mice expressing -1058FGFp.luc, a significant three- to sevenfold stimulation of FGF-2 promoter activitywas detected in the hearts of two independent lines 6 h after intraperitoneal administration of phenylephrine (50 mg/kg). This increase was still apparent at 24 h but was not detected at 48 h posttreatment. Analysis of FGF-2mRNA in normal mouse hearts revealed accumulation of the 6.1-kb transcriptat 24 h. Control of local FGF-2 synthesis at the transcriptional level through adrenergic stimulation may be important in the response to injury as well as in the maintenance of a healthy myocardium.

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Documento generato il 28/09/20 alle ore 11:52:33